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Patients in this study were diagnosed as having colon cancer through histological examination of each tumor tissue by biopsy. Blotting index of serum \u03b2\u2010haptoglobin with <jats:italic>Aleuria aurantia<\/jats:italic> lectin (AAL) was clearly higher for cancer patients than for healthy subjects. No such distinction was observed for blotting with three other lectins and two monoclonal antibodies. To determine tumor\u2010associated reactivity of AAL binding as compared to inflammatory processes in colonic tissues, \u03b2\u2010haptoglobin separated from sera of 5 patients with Crohn's disease (CD), and 4 patients with ulcerative colitis (UC), was studied. All these cases, except one case of UC, showed AAL index lower than that in cancer cases, similarly to healthy subjects. The higher AAL binding of \u03b2\u2010haptoglobin in colon cancer patients than in healthy subjects appeared to be due to \u03b1\u2010L\u2010fucosyl residue, since it was eliminated by bovine kidney \u03b1\u2010fucosidase treatment. N\u2010linked glycans of serum haptoglobin from colon cancer patients <jats:italic>vs<\/jats:italic>. healthy subjects were released by N\u2010glycanase, fluorescence\u2010labeled, and subjected to normal\u2010phase high performance liquid chromatography (NP\u2010HPLC). Glycan structures were determined based on glucose unit (GU) values and their changes upon sequential treatment with various exoglycosidases. Glycosyl sequences and their branching status of glycans from 14 cases of serum \u03b2\u2010haptoglobin were characterized. The identified glycans were sialylated or nonsialylated, bi\u2010antennary or tri\u2010antennary structures, with or without terminal fucosylation.<\/jats:p>","DOI":"10.1002\/ijc.24685","type":"journal-article","created":{"date-parts":[[2009,6,23]],"date-time":"2009-06-23T18:16:26Z","timestamp":1245780986000},"page":"142-155","source":"Crossref","is-referenced-by-count":45,"title":["N\u2010glycosylation status of \u03b2\u2010haptoglobin in sera of patients with colon cancer, chronic inflammatory diseases and normal 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