{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,25]],"date-time":"2026-05-25T15:05:04Z","timestamp":1779721504890,"version":"3.53.1"},"reference-count":37,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2009,7,7]],"date-time":"2009-07-07T00:00:00Z","timestamp":1246924800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2009,8,24]],"date-time":"2009-08-24T00:00:00Z","timestamp":1251072000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2010,1,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Cancer cells undergo significant changes in carbohydrate expression, and these alterations can be useful as biomarkers and therapeutic targets. In this study, we investigated the expression of carbohydrate antigens containing a terminal GalNAc\u03b11\u20103Gal or GalNAc\u03b11\u20106Gal on human cervix and cervical carcinoma. Monoclonal antibodies to each of these carbohydrates were generated by immunizing rabbits with the corresponding antigen conjugated to KLH followed by hybridoma production. Antibodies were screened and evaluated using a combination of carbohydrate microarray profiling, ELISA, dot blot and immunohistochemical staining to verify specificity. Antibody 132\u20103 was found to selectively recognize GalNAc\u03b11\u20103Gal with little cross\u2010reactivity to other structurally similar antigens such as GalNAc\u03b11\u20106Gal, blood group A, Forssman antigen and the Tn antigen on both solution assays and human tissue. Although GalNAc\u03b11\u20106Gal expression was not detected, GalNAc\u03b11\u20103Gal expression was found on 55% of squamous cell carcinomas. Expression in normal tissue was observed but was restricted to the suprabasal epithelial layer. Importantly, we found expression of the antigen on cervical cancer had a statistically significant correlation with the 5\u2010year survival rate of the patients (48\n                    <jats:italic>vs.<\/jats:italic>\n                    85% for antigen negative\n                    <jats:italic>vs.<\/jats:italic>\n                    positive,\n                    <jats:italic>p<\/jats:italic>\n                    = 0.017). Expression of GalNAc\u03b11\u20103Gal did not correlate with other clinical factors including tumor stage, size and lymph node metastasis, indicating the antigen is a new, independent biomarker for the prognosis of cervical cancer. Published 2009 UICC.\n                  <\/jats:p>","DOI":"10.1002\/ijc.24716","type":"journal-article","created":{"date-parts":[[2009,7,9]],"date-time":"2009-07-09T07:52:38Z","timestamp":1247125958000},"page":"459-468","source":"Crossref","is-referenced-by-count":30,"title":["GalNAc\u03b11\u20103Gal, a new prognostic marker for cervical cancer"],"prefix":"10.1002","volume":"126","author":[{"given":"Qian","family":"Li","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miriam R.","family":"Anver","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhitao","family":"Li","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Donna O.","family":"Butcher","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey C.","family":"Gildersleeve","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,8,24]]},"reference":[{"key":"e_1_2_6_2_2","volume-title":"Comprehensive cervical cancer control: a guide to essential practice","year":"2006"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.3322\/canjclin.57.1.43"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2008.01.008"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-07-4085"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-07-4554"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1649"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrd1751"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1053\/tmrv.2000.16229"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/s001090050144"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.6729450"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(19930701)72:1<154::AID-CNCR2820720129>3.0.CO;2-C"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-3835(93)90239-6"},{"key":"e_1_2_6_14_2","first-page":"2229","article-title":"Expression of Tn and sialyl\u2010Tn antigens in the neoplastic transformation of uterine cervical epithelial cells","volume":"56","author":"Terasawa K","year":"1996","journal-title":"Cancer Res"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1007\/s004280000218"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/cbic.200500165"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/anie.200600591"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwm047"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1021\/pr9002245"},{"key":"e_1_2_6_20_2","first-page":"971","article-title":"Resolving conflicting data on expression of the Tn antigen and implications for clinical trials with cancer vaccines","volume":"8","author":"Li Q","year":"2009","journal-title":"Mol Cancer Res"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)39465-6"},{"key":"e_1_2_6_22_2","first-page":"4185","article-title":"Blood group A\u2010like glycolipid and a novel Forssman antigen in the hepatocarcinoma of a blood group O individual","volume":"41","author":"Yokota M","year":"1981","journal-title":"Cancer Res"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(17)38055-9"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.20.9348"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1039\/b800725j"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1021\/bc800153t"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cbpa.2008.01.031"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1586\/14760584.6.6.957"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/nchembio785"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1002\/qsar.200640081"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3700268"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1021\/bi00631a025"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00731275"},{"key":"e_1_2_6_34_2","first-page":"1357","article-title":"Carbohydrate antigens: synthesis aspects and immunological applications in cancer. 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