{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T17:49:52Z","timestamp":1778953792320,"version":"3.51.4"},"reference-count":49,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2009,7,16]],"date-time":"2009-07-16T00:00:00Z","timestamp":1247702400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2009,12,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Cervical cancer is the second most common type of cancer in women worldwide. Preinvasive disease can be detected by cervical cytology. All currently available cytology technologies rely on the visual analysis of exfoliated cells from the uterine cervix. Improvement of conventional cytological screening has been proposed by the introduction of molecular\u2010based markers applied to liquid\u2010based cytology (LBC), the suspension of cells collected from the cervix. DNA methylation changes occur very early in carcinogenesis and identification of appropriate DNA methylation markers in such samples should be able to distinguish high\u2010grade squamous intraepithelial lesions (HSIL) from nonspecific cytology changes and the normal cervix. To address this potential, we have undertaken a proof\u2010of\u2010principle study of methylation status of LBC samples from HSIL cytology cases compared against matched normal controls. Using quantitative methylation\u2010specific PCR on 28 genes, we found <jats:italic>SOX1<\/jats:italic>, <jats:italic>HOXA11<\/jats:italic> and <jats:italic>CADM1<\/jats:italic> to significantly discriminate between the groups analyzed (<jats:italic>p<\/jats:italic> &lt; 0.01). Area under the receiver operating characteristic (ROC) curve (AUC) demonstrated that methylation of <jats:italic>SOX1<\/jats:italic>, <jats:italic>HOXA11<\/jats:italic> and <jats:italic>CADM1<\/jats:italic> could discriminate between HSIL cases and controls with high sensitivity and specificity (AUC 0.910, 0.844 and 0.760, respectively). The results were further validated in an independent set. This proof\u2010of\u2010principle study is the first to validate the results in an independent case\/control set and presents <jats:italic>HOXA11<\/jats:italic>, a gene that is important for cervical development, as a potentially useful DNA marker in LBC samples. Further assessment of these preliminary estimates will need to be performed in a larger cohort to confirm clinical utility. \u00a9 2009 UICC<\/jats:p>","DOI":"10.1002\/ijc.24745","type":"journal-article","created":{"date-parts":[[2009,7,17]],"date-time":"2009-07-17T16:59:16Z","timestamp":1247849956000},"page":"2995-3002","source":"Crossref","is-referenced-by-count":50,"title":["DNA methylation analysis in liquid\u2010based cytology for cervical cancer 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