{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T21:42:18Z","timestamp":1791409338929,"version":"4.3.3"},"reference-count":36,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2009,8,6]],"date-time":"2009-08-06T00:00:00Z","timestamp":1249516800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2010,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Recent research advances show that tumor cell intravasation (entry into the circulation) and metastasis occur very early in breast cancer progression. Clinical studies also illustrate the potential importance of detection of circulating tumor cells (CTCs) in outcomes of patients with metastatic breast cancer. Whether these cells exhibit the invasiveness and express tumor stem or progenitor markers, hallmark of the metastatic phenotype, is less well characterized. To detect CTCs with the invasive phenotype and to explore their molecular features, we applied a functional cell separation method, called collagen adhesion matrix (CAM) assay, as enrichment and identification steps. The CAM\u2010coated device successfully recovered tumor cells spiked in 1 ml of blood with a 54% \u00b1 9% (\n                    <jats:italic>n<\/jats:italic>\n                    = 18) recovery rate and 0.5\u201335% purity, and detected invasive tumor cells in 10\/10 blood samples (100% yield) from patients with metastatic breast cancer with a range of 18\u2013256 CTCs\/ml and average of 126 \u00b1 25 (mean \u00b1 SD) CTCs\/ml. CTCs were detected in blood samples of 28\/54 (52%) Stage I\u2013III breast cancer patients with a mean count of 61 CTCs\/ml. Furthermore, the relative frequency of these cells correlated to the staging, lymph node\u2010status and survival of patients with early stage breast cancer. CAM\u2010captured cells were capable of propagation in culture. Gene expression and multiplex flow cytometric analyses on CAM\u2010captured cells demonstrated the existence of distinct populations of CTCs including these of epithelial lineage and stem or progenitor cells. Thus, CAM\u2010initiated CTC detection provides advantages for examining invasiveness and tumor progenitor phenotypes.\n                  <\/jats:p>","DOI":"10.1002\/ijc.24814","type":"journal-article","created":{"date-parts":[[2009,8,6]],"date-time":"2009-08-06T14:02:19Z","timestamp":1249567339000},"page":"669-683","source":"Crossref","is-referenced-by-count":140,"title":["Isolation of circulating epithelial and tumor progenitor cells with an invasive phenotype from breast cancer patients"],"prefix":"10.1002","volume":"126","author":[{"given":"Janice","family":"Lu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tina","family":"Fan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiang","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Zeng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eva","family":"Zaslavsky","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John J.","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael A.","family":"Frohman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marc G.","family":"Golightly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Madajewicz","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen\u2010Tien","family":"Chen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,9,23]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2007.12.003"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1161621"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63570-5"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djn419"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2375"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2006.12.014"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-07-4758"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-07-0419"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1835"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2008.207"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2007.01.013"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2008.03.027"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1912"},{"key":"e_1_2_6_15_2","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1038\/71429","article-title":"Intravascular origin of metastasis from the proliferation of endothelium\u2010attached tumor cells: a new model for metastasis","volume":"6","author":"Al Mehdi AB","year":"2000","journal-title":"Nat Med"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200309112"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/ajcp\/112.2.171"},{"key":"e_1_2_6_18_2","first-page":"2598","article-title":"Rare expression of epithelial cell adhesion molecule on residual micrometastatic breast cancer cells after adjuvant chemotherapy","volume":"9","author":"Thurm H","year":"2003","journal-title":"Clin Cancer Res"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.20391"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(04)01381-6"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.08.006"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2008.02.066"},{"key":"e_1_2_6_23_2","article-title":"Dependence of invadopodia function on collagen fiber spacing and crosslinking: computational modeling and experimental evidence","author":"Enderling H","journal-title":"Biophys J"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.35.24947"},{"key":"e_1_2_6_25_2","doi-asserted-by":"crossref","first-page":"27","DOI":"10.1002\/ijc.23871","article-title":"Elevation of seprase expression and promotion of an invasive phenotype by collagenous matrices in ovarian tumor cells","volume":"124","author":"Kennedy A","year":"2008","journal-title":"Int J Cancer"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ygyno.2008.09.021"},{"key":"e_1_2_6_27_2","article-title":"Functional phenotyping and genotyping of circulating tumor cells from patients with castration resistant prostate cancer","author":"Paris PL","journal-title":"Cancer Lett"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1213\/00000539-199406000-00017"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-0102-03"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-1058"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-04-0378"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature06385"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-03-0361"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0404036101"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.042372199"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa040766"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10549-006-9181-4"}],"container-title":["International Journal of Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fijc.24814","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijc.24814","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T06:00:46Z","timestamp":1697436046000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ijc.24814"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,9,23]]},"references-count":36,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2010,2]]}},"alternative-id":["10.1002\/ijc.24814"],"URL":"https:\/\/doi.org\/10.1002\/ijc.24814","archive":["Portico"],"relation":{},"ISSN":["0020-7136","1097-0215"],"issn-type":[{"value":"0020-7136","type":"print"},{"value":"1097-0215","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,9,23]]}}}