{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T07:26:42Z","timestamp":1778657202849,"version":"3.51.4"},"reference-count":28,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2009,8,26]],"date-time":"2009-08-26T00:00:00Z","timestamp":1251244800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2010,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Several mouse models studying the <jats:italic>MLL<\/jats:italic> fusion\u2010induced leukemic transformation showed that a myeloproliferation stage precedes leukemia or occurred as the only phenotype of hematological disorder in mice. We established 6 <jats:italic>MLL\/AF10(OM\u2010LZ<\/jats:italic>)\u2010immortalized cell lines by retrovirally transducing the fusion gene into bone marrow cells from B6 or congenic GFP\u2010B6 mice. Immunophenotypic and cytological analyses revealed that the immortalized cell lines could be divided into 2 types. Type I had a high percentage of cells expressing monocytic lineage marker CD115 in the medium containing IL3 and could terminally differentiate into granulocytes and monocytes in response to granulocyte colony\u2010stimulating factor (G\u2010CSF) and macrophage colony\u2010stimulating factor (M\u2010CSF) treatments, respectively. On the other hand, type II had a low percentage of cells expressing CD115. The type II cell lines could not differentiate into granulocytes by G\u2010CSF treatment and died rapidly in response to M\u2010CSF treatment. Transplantation of both types I and II cells induced lethal myeloproliferative disease (MPD)\u2010like myeloid leukemia in most of the sublethally irradiated B6 mice. Flow cytometric analysis of GFP and lineage markers of the peripheral blood cells from MPD mice revealed that the monocytes and granulocytes were generated not only from the donor cells but also from the host cells. RT\u2010PCR analysis revealed that the <jats:italic>MLL\/AF10(OM\u2010LZ<\/jats:italic>)\u2010immortalized cells expressed mRNAs encoding colony\u2010stimulating factors (CSFs) of M\u2010CSF and GM\u2010CSF and inflammatory cytokines of IL\u20101\u03b1, IL\u20101\u03b2 and TNF\u2010\u03b1. Our results showed that the <jats:italic>MLL\/AF10(OM\u2010LZ<\/jats:italic>)\u2010immortalized cells could induce host cell proliferation in the transplanted mice, probably through stimulation by CSFs or cytokines produced by the donor cells.<\/jats:p>","DOI":"10.1002\/ijc.24867","type":"journal-article","created":{"date-parts":[[2009,8,27]],"date-time":"2009-08-27T03:44:43Z","timestamp":1251344683000},"page":"1621-1629","source":"Crossref","is-referenced-by-count":10,"title":["<i>MLL\/AF10(OM\u2010LZ)<\/i>\u2010immortalized cells expressed cytokines and induced host cell proliferation in a mouse bone marrow transplantation model"],"prefix":"10.1002","volume":"126","author":[{"given":"Jen\u2010Fen","family":"Fu","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng\u2010Lung","family":"Hsu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lee\u2010Yung","family":"Shih","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,1,28]]},"reference":[{"key":"e_1_2_6_2_2","volume-title":"Cancer cytogenetics: chromosomal and molecular aberrations of tumor cells","author":"Heim S","year":"1995"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2253"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0950-3536(11)80050-8"},{"key":"e_1_2_6_5_2","first-page":"3800","article-title":"Southern blot analysis of ALL\u20101 rearrangements at chromosome 11q23 in acute leukemia","volume":"53","author":"Coco FL","year":"1993","journal-title":"Cancer Res"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI116978"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V84.11.3776.bloodjournal84113776"},{"key":"e_1_2_6_8_2","first-page":"4220","article-title":"Breakpoint heterogeneity in t(10;11) translocation in AML\u2010M4\/M5 resulting in fusion of AF10 and MLL is resolved by fluorescent in situ hybridization analysis","volume":"55","author":"Beverloo HB","year":"1995","journal-title":"Cancer Res"},{"key":"e_1_2_6_9_2","doi-asserted-by":"crossref","first-page":"2073","DOI":"10.1182\/blood.V86.6.2073.bloodjournal8662073","article-title":"The t(10;11) translocation in acute myeloid leukemia (M5) consistently fuses the leucine zipper motif of AF10 on to the HRX gene","volume":"86","author":"Chaplin T","year":"1995","journal-title":"Blood"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2404024"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V99.10.3780"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.22.18.6542-6552.2002"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V100.1.238"},{"key":"e_1_2_6_14_2","doi-asserted-by":"crossref","first-page":"4543","DOI":"10.1182\/blood.V91.12.4543","article-title":"A new cytokine\u2010dependent monoblastic cell line with t(9;11)(p22;q23) differentiates to macrophages with macrophage colony\u2010stimulating factor (M\u2010CSF) and to osteoclast\u2010like cells with M\u2010CSF and interleukin\u20104","volume":"91","author":"Ikeda T","year":"1998","journal-title":"Blood"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1207125"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/16.14.4226"},{"key":"e_1_2_6_17_2","first-page":"6480","article-title":"MLL\u2010ENL causes a reversible and myc\u2010dependent block of myelomonocytic cell differentiation","volume":"61","author":"Schreiner S","year":"2001","journal-title":"Cancer Res"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-05-1691"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.dci.2003.09.010"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V70.5.1700.1700"},{"key":"e_1_2_6_21_2","doi-asserted-by":"crossref","first-page":"201","DOI":"10.4049\/jimmunol.141.1.201","article-title":"Enhancement of release of granulocyte\u2010 and granulocyte\u2010macrophage colony\u2010stimulating factors from phytohemagglutinin\u2010stimulated sorted subsets of human T lymphocytes by recombinant human tumor necrosis factor\u2010alpha. Synergism with recombinant human IFN\u2010gamma","volume":"141","author":"Lu L","year":"1988","journal-title":"J Immunol"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V70.1.55.55"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/j.1460-2075.1987.tb02499.x"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113316"},{"key":"e_1_2_6_25_2","first-page":"785","article-title":"Interferon\u2010gamma enhances the LPS\u2010induced G\u2010CSF gene expression in human adherent monocytes, which is regulated at transcriptional and posttranscriptional levels","volume":"21","author":"de Wit H","year":"1993","journal-title":"Exp Hematol"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200522725"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.13.3564"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600521"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/19.17.4655"}],"container-title":["International Journal of Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fijc.24867","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijc.24867","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T10:07:36Z","timestamp":1697450856000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ijc.24867"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,1,28]]},"references-count":28,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2010,4]]}},"alternative-id":["10.1002\/ijc.24867"],"URL":"https:\/\/doi.org\/10.1002\/ijc.24867","archive":["Portico"],"relation":{},"ISSN":["0020-7136","1097-0215"],"issn-type":[{"value":"0020-7136","type":"print"},{"value":"1097-0215","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,1,28]]}}}