{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T06:49:52Z","timestamp":1767854992202,"version":"3.49.0"},"reference-count":40,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2009,10,8]],"date-time":"2009-10-08T00:00:00Z","timestamp":1254960000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2010,5,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Oral squamous cell carcinomas (OSCC) are malignant tumors with a potent activity of local bone invasion; however, the molecular mechanisms of tumor osteolysis are unclear. In this study, we identified high level expression of chemokine ligand, CXCL13 and RANK ligand (RANKL) in OSCC cells (SCC1, SCC12 and SCC14a). OSCC cell\u2010conditioned media (20%) induced osteoclast differentiation which was inhibited by OPG in peripheral blood monocyte cultures indicating that OSCC cells produce soluble RANKL. Recombinant hCXCL13 (10 ng\/ml) significantly enhanced RANKL\u2010stimulated osteoclast differentiation in these cultures. Trans\u2010well migration assay identified that CXCL13 induces chemotaxis of peripheral blood monocytes <jats:italic>in vitro<\/jats:italic> which was inhibited by addition of anti\u2010CXCR5 receptor antibody. Zymogram analysis of conditioned media from OSCC cells revealed matrix metalloproteinase\u20109 (MMP\u20109) activity. Interestingly, CXCL13 treatment to OSCC cells induced CXCR5 and MMP\u20109 expression suggesting an autocrine regulatory function in OSCC cells. To examine the OSCC tumor cell bone invasion\/osteolysis, we established an <jats:italic>in vivo<\/jats:italic> model for OSCC by subcutaneous injection of OSCC cells onto the surface of calvaria in NCr\u2010nu\/nu athymic mice, which developed tumors in 4\u20135 weeks. \u03bcCT analysis revealed numerous osteolytic lesions in calvaria from OSCC tumor\u2010bearing mice. Histochemical staining of calvarial sections from these mice revealed a significant increase in the numbers of TRAP\u2010positive osteoclasts at the tumor\u2010bone interface. Immunohistochemical analysis confirmed CXCL13 and MMP\u20109 expression in tumor cells. Thus, our data implicate a functional role for CXCL13 in bone invasion and may be a potential therapeutic target to prevent osteolysis associated with OSCC tumors <jats:italic>in vivo<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/ijc.24920","type":"journal-article","created":{"date-parts":[[2009,10,8]],"date-time":"2009-10-08T17:52:11Z","timestamp":1255024331000},"page":"2319-2329","source":"Crossref","is-referenced-by-count":36,"title":["Role of CXC chemokine ligand 13 in oral squamous cell carcinoma associated osteolysis in athymic mice"],"prefix":"10.1002","volume":"126","author":[{"given":"Subramanya N.M.","family":"Pandruvada","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sambandam","family":"Yuvaraj","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiang","family":"Liu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kumaran","family":"Sundaram","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Srinivasan","family":"Shanmugarajan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William L.","family":"Ries","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James S.","family":"Norris","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven D.","family":"London","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sakamuri V.","family":"Reddy","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,12,22]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/hed.10004"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1177\/154405910808700104"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-05-0301"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cgt.7700951"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2006.09.005"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-06-0558"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.7.3540"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1615\/CritRevEukaryotGeneExpr.v14.i4.20"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2006.03.015"},{"key":"e_1_2_6_11_2","first-page":"663","article-title":"Oral squamous cell carcinomas stimulate osteoclast differentiation","volume":"20","author":"Deyama Y","year":"2008","journal-title":"Oncol Rep"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80165-X"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.68.1.1"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/35065016"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.21514"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.187.4.655"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-06-0535"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2005.11.001"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-2-6"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/path.2241"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2005.01.021"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2006.10.001"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2008.10.011"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-05-4398"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2008.09.015"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncponc0381"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1158\/1541-7786.MCR-07-0208"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-003-0587-3"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2007.12.002"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-4827(03)00344-6"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djn059"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2003.09.030"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.10188"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.20435"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-04-0852"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.21008"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgn045"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.130.8.929"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1368-8375(02)00006-4"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2005.04.013"},{"key":"e_1_2_6_41_2","doi-asserted-by":"publisher","DOI":"10.1097\/MLG.0b013e318174e09b"}],"container-title":["International Journal of Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fijc.24920","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijc.24920","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T04:56:42Z","timestamp":1697432202000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ijc.24920"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,12,22]]},"references-count":40,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2010,5,15]]}},"alternative-id":["10.1002\/ijc.24920"],"URL":"https:\/\/doi.org\/10.1002\/ijc.24920","archive":["Portico"],"relation":{},"ISSN":["0020-7136","1097-0215"],"issn-type":[{"value":"0020-7136","type":"print"},{"value":"1097-0215","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,12,22]]}}}