{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T15:13:45Z","timestamp":1790176425105,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2009,12,3]],"date-time":"2009-12-03T00:00:00Z","timestamp":1259798400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2010,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study aimed to evaluate whether quantitation of high\u2010risk human papillomavirus (HR\u2010HPV) E6 messenger RNA (mRNA) can be a potential biomarker for detecting the severity of cervical lesions. HPV genotyping was performed using a modified MY11\/GP6+ PCR for HPV DNA amplification, followed by HPV genotype\u2010specific hybridization with on a gene chip. E6 type\u2010specific PCR was used to validate multiple infections. Quantitative real\u2010time reverse transcriptase (QRT\u2010PCR) and real\u2010time PCR used to measure mRNA levels and DNA viral loads of 6 HPV oncogenic types (HPV 16, 18, 31, 33, 52 and 58) in 720 liquid\u2010based cytology samples. The HPV DNA and RNA measurements were correlated with cervical lesions diagnosed by histopathologic examination. mRNA transcripts in the 6 types HPV DNA\u2010positive cases was lower in normal women and &lt;CIN 1 (23%), women with CIN 1 (54%), CIN2+ (77%) and CIN3+ (80%) (<jats:italic>p<\/jats:italic> &lt; 0.001). Geometric mean mRNA levels ranged from 24.5 (copies per 50 ng total RNA) in normal women and &lt;CIN 1 to 210.8 in those with CIN 1, 629.0 in CIN2+ and 699.0 in CIN3+ (<jats:italic>p<\/jats:italic> &lt; 0.0001). Trends of increasing viral mRNA with severity of histopathologic diagnosis were significant for HPV 16, 18, 52 and 58 transcripts but not for HPV 31 and 33 transcripts. However, geometric mean DNA viral loads of HPV 16, 18, 52 and 58 DNA did not significantly increase with the severity of cervical dysplasia. Therefore, quantitative HPV E6 mRNA levels of high\u2010risk HPV types are potentially useful biomarkers for distinguishing among HPV infections, cervical precancerous lesions and cancer.<\/jats:p>","DOI":"10.1002\/ijc.25078","type":"journal-article","created":{"date-parts":[[2009,12,3]],"date-time":"2009-12-03T13:16:49Z","timestamp":1259846209000},"page":"622-632","source":"Crossref","is-referenced-by-count":38,"title":["Type\u2010specific human papillomavirus oncogene messenger RNA levels correlate with the severity of cervical neoplasia"],"prefix":"10.1002","volume":"127","author":[{"given":"Chih\u2010Ming","family":"Ho","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bor\u2010Heng","family":"Lee","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shwu\u2010Fen","family":"Chang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsai\u2010Yen","family":"Chien","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shih\u2010Hung","family":"Huang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiu\u2010Cho","family":"Yan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen\u2010Fang","family":"Cheng","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,5,24]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/87.11.796"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/92.9.690"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.283.1.87"},{"key":"e_1_2_5_5_2","doi-asserted-by":"crossref","first-page":"2811","DOI":"10.1128\/jvi.68.5.2811-2821.1994","article-title":"Reversible 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