{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T02:25:09Z","timestamp":1785896709522,"version":"3.56.0"},"reference-count":31,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2009,12,16]],"date-time":"2009-12-16T00:00:00Z","timestamp":1260921600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2010,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We studied orchiectomy specimens from 130 patients immuhistochemically with testicular germ cell tumor (TGCT) using anti\u2010core 2 <jats:italic>N<\/jats:italic>\u2010acetylglucosaminyltransferase\u20101 (C2GnT\u20101) antibody. The incidence of C2GnT\u20101 positivity in stage I disease (29.5%, 21\/71) was significantly lower than that in higher stages (84.7%, 50\/59) (<jats:italic>P<\/jats:italic> &lt; 0.001, \u03c7<jats:sup>2<\/jats:sup> test). This significant difference was also found when the cases were divided into seminoma and NSGCT according to histopathological classification. Kaplan\u2010Meier plots and the log rank test showed that in the patients with stage I seminoma, C2GnT\u20101\u2010positive cases had a higher risk for recurrence (<jats:italic>P<\/jats:italic> &lt; 0.001). This was also the case with the patients with stage I NSGCT (<jats:italic>P<\/jats:italic> &lt; 0.001). To determine whether C2GnT\u20101 promotes aggressive behavior of cancer cells, a C2GnT\u20101\u2010negative human TGCT cell line, JKT\u20101, was stably transfected with a mammalian expression vector containing C2GnT\u20101 cDNA. <jats:italic>In vitro<\/jats:italic> assays revealed that JKT\u20101\u2010C2 cells are more invasive than mock transfectants, although there are no differences in proliferation activity. When orthotopically inoculated into athymic nude mice, JKT\u20101\u2010C2 cells produced larger testicular tumors extending to the retroperitoneum with mesenteric metastasis, while mock transfectants produced small tumors without metastasis (<jats:italic>P<\/jats:italic> &lt; 0.01, Mann\u2010Whitney's <jats:italic>U<\/jats:italic>\u2010test). When injected <jats:italic>via<\/jats:italic> the tail vein, JKT\u20101\u2010C2 cells produced a number of metastatic lung foci. In contrast, mock transfectants produced a small number of nodules (<jats:italic>p<\/jats:italic> &lt; 0.01, Mann\u2010Whitney's <jats:italic>U<\/jats:italic>\u2010test). These results strongly suggest that C2GnT\u20101 enhances the metastatic potential of TGCT and may be a reliable biomarker for aggressive potential of TGCT.<\/jats:p>","DOI":"10.1002\/ijc.25117","type":"journal-article","created":{"date-parts":[[2009,12,16]],"date-time":"2009-12-16T16:03:53Z","timestamp":1260979433000},"page":"1052-1059","source":"Crossref","is-referenced-by-count":40,"title":["Core 2 <i>N<\/i>\u2010acetylglucosaminyltransferase\u20101 expression induces aggressive potential of testicular germ cell 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