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The molecular markers CD24, CD44, CD34, and epithelial cell adhesion molecule (EpCAM) are widely used, individually or in combination, to characterize some types of CSCs. In this study, we used these markers to identify a subpopulation of cells in extrahepatic cholangiocarcinoma (ECC) with cancer stem\/progenitor cell\u2010like properties. We found that CD24<jats:sup>+<\/jats:sup>CD44<jats:sup>+<\/jats:sup>EpCAM<jats:sup>high<\/jats:sup> cells (0.39\u20132.27%) were present in human ECC tissues. The expression of a CD24<jats:sup>+<\/jats:sup>CD44<jats:sup>+<\/jats:sup>EpCAM<jats:sup>high<\/jats:sup> subpopulation was consistent with primary cancers and could be duplicated during serial <jats:italic>in vivo<\/jats:italic> passaging in NOD\/SCID mice. CD24<jats:sup>+<\/jats:sup>CD44<jats:sup>+<\/jats:sup>EpCAM<jats:sup>high<\/jats:sup> cells isolated from 3 cholangiocarcinoma xenografts showed high tumorigenic potential compared with CD24<jats:sup>\u2212<\/jats:sup>CD44<jats:sup>\u2212<\/jats:sup>EpCAM<jats:sup>low\/\u2212<\/jats:sup> cells. These tumorigenic ECC cells exhibited the stem cell properties of self\u2010renewal and ability to produce heterogeneous progeny. We report the identification of a CSC population in ECC characterized by CD24, CD44 and EpCAM phenotypes. Our findings could provide new insight into the tumorigenesis of cholangiocarcinoma and offer a potential target for anti\u2010cancer therapy.<\/jats:p>","DOI":"10.1002\/ijc.25317","type":"journal-article","created":{"date-parts":[[2010,3,15]],"date-time":"2010-03-15T15:25:16Z","timestamp":1268666716000},"page":"72-81","source":"Crossref","is-referenced-by-count":47,"title":["Isolation and characterization of tumorigenic extrahepatic cholangiocarcinoma cells with stem cell\u2010like 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