{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T13:01:30Z","timestamp":1790859690596,"version":"4.1.0"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2010,4,19]],"date-time":"2010-04-19T00:00:00Z","timestamp":1271635200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2011,2,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Bevacizumab is a humanized monoclonal antibody that binds to tumor\u2010secreted vascular endothelial growth factor (VEGF)\u2010A and inhibits tumor angiogenesis. In 2004, the antibody was approved by the US Food and Drug Administration (FDA) for the treatment of metastatic colorectal carcinoma in combination with chemotherapy. This report describes the preclinical evaluation of a radioimmunoconjugate, <jats:sup>86<\/jats:sup>Y\u2010CHX\u2010A\u2033\u2010DTPA\u2010bevacizumab, for potential use in Positron Emission Tomography (PET) imaging of VEGF\u2010A tumor angiogenesis and as a surrogate marker for <jats:sup>90<\/jats:sup>Y\u2010based radioimmunotherapy. Bevacizumab was conjugated to CHX\u2010A\u2033\u2010DTPA and radiolabeled with <jats:sup>86<\/jats:sup>Y. <jats:italic>In vivo<\/jats:italic> biodistribution and PET imaging studies were performed on mice bearing VEGF\u2010A\u2013secreting human colorectal (LS\u2010174T), human ovarian (SKOV\u20103) and VEGF\u2010A\u2013negative human mesothelioma (MSTO\u2010211H) xenografts. Biodistribution and PET imaging studies demonstrated highly specific tumor uptake of the radioimmunoconjugate. In mice bearing VEGF\u2010A\u2013secreting LS\u2010174T, SKOV\u20103 and VEGF\u2010A\u2013negative MSTO\u2010211H tumors, the tumor uptake at 3 days postinjection was 13.6 \u00b1 1.5, 17.4 \u00b1 1.7 and 6.8 \u00b1 0.7 % ID\/g, respectively. The corresponding tumor uptake in mice coinjected with 0.05 mg cold bevacizumab were 5.8 \u00b1 1.3, 8.9 \u00b1 1.9 and 7.4 \u00b1 1.0 % ID\/g, respectively at the same time point, demonstrating specific blockage of the target in VEGF\u2010A\u2013secreting tumors. The LS\u2010174T and SKOV3 tumors were clearly visualized by PET imaging after injecting 1.8\u20132.0 MBq <jats:sup>86<\/jats:sup>Y\u2010CHX\u2010A\u2033\u2010DTPA\u2010bevacizumab. Organ uptake quantified by PET closely correlated (<jats:italic>r<\/jats:italic><jats:sup>2<\/jats:sup> = 0.87, <jats:italic>p<\/jats:italic> = 0.64, <jats:italic>n<\/jats:italic> = 18) to values determined by biodistribution studies. This preclinical study demonstrates the potential of the radioimmunoconjugate, <jats:sup>86<\/jats:sup>Y\u2010CHX\u2010A\u2033\u2010DTPA\u2010bevacizumab, for noninvasive assessment of the VEGF\u2010A tumor angiogenesis status and as a surrogate marker for <jats:sup>90<\/jats:sup>Y\u2010CHX\u2010A\u2033\u2010DTPA\u2010bevacizumab radioimmunotherapy.<\/jats:p>","DOI":"10.1002\/ijc.25409","type":"journal-article","created":{"date-parts":[[2010,4,19]],"date-time":"2010-04-19T18:25:39Z","timestamp":1271701539000},"page":"920-926","source":"Crossref","is-referenced-by-count":55,"title":["PET imaging of tumor angiogenesis in mice with VEGF\u2010A\u2013targeted <sup>86<\/sup>Y\u2010CHX\u2010A\u2033\u2010DTPA\u2010bevacizumab"],"prefix":"10.1002","volume":"128","author":[{"given":"Tapan K.","family":"Nayak","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kayhan","family":"Garmestani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwamena E.","family":"Baidoo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diane E.","family":"Milenic","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin W.","family":"Brechbiel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,12,22]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81683-9"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature04483"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nm0603-669"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1053\/sonc.2002.37263"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrd1381"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/nrclinonc.2009.110"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-09-0731"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/jmri.21710"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/neuonc\/nop027"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.2967\/jnumed.107.041301"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.23404"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2008.05.026"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-8051(02)00322-0"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.2967\/jnumed.109.071290"},{"key":"e_1_2_5_16_2","article-title":"PET imaging of HER1\u2010expressing xenografts in mice with (86)Y\u2010CHX\u2010A\u2033\u2010DTPA\u2010cetuximab","author":"Nayak TK","journal-title":"Eur J Nucl Med Mol Imaging"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1021\/bc800299f"},{"key":"e_1_2_5_18_2","first-page":"1148","article-title":"Pharmacokinetics and Biodistribution of (86)Y\u2010Trastuzumab for (90)Y dosimetry in an ovarian carcinoma model: correlative MicroPET and MRI","volume":"44","author":"Palm S","year":"2003","journal-title":"J Nucl Med"},{"key":"e_1_2_5_19_2","doi-asserted-by":"publisher","DOI":"10.1021\/bc00016a014"},{"key":"e_1_2_5_20_2","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-07-0501"},{"key":"e_1_2_5_21_2","first-page":"891","article-title":"Vascularization of the Brown Pearce rabbit epithelioma transplant as seen in the transparent ear chamber","volume":"42","author":"Ide AG","year":"1939","journal-title":"Am J Roentgenol"},{"key":"e_1_2_5_22_2","first-page":"159","article-title":"Growth and vascularization of transplanted mouse melanomas","volume":"4","author":"Algire GH","year":"1948","journal-title":"Ann N Y Acad Sci"},{"key":"e_1_2_5_23_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197111182852108"},{"key":"e_1_2_5_24_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa032691"},{"key":"e_1_2_5_25_2","doi-asserted-by":"publisher","DOI":"10.1089\/cbr.2008.0574"},{"key":"e_1_2_5_26_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00259-004-1561-6"},{"key":"e_1_2_5_27_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00259-006-0160-0"},{"key":"e_1_2_5_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.nucmedbio.2009.01.019"},{"key":"e_1_2_5_29_2","first-page":"159","article-title":"PET imaging problems with the non\u2010standard positron emitters Yttrium\u201086 and Iodine\u2010124","volume":"52","author":"Herzog H","year":"2008","journal-title":"Q J Nucl Med Mol Imaging"},{"key":"e_1_2_5_30_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00259-002-1112-y"}],"container-title":["International Journal of Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fijc.25409","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijc.25409","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,16]],"date-time":"2023-10-16T05:07:48Z","timestamp":1697432868000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ijc.25409"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,12,22]]},"references-count":29,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2011,2,15]]}},"alternative-id":["10.1002\/ijc.25409"],"URL":"https:\/\/doi.org\/10.1002\/ijc.25409","archive":["Portico"],"relation":{},"ISSN":["0020-7136","1097-0215"],"issn-type":[{"value":"0020-7136","type":"print"},{"value":"1097-0215","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,12,22]]}}}