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In our study, we have sought to identify novel genes involved in the pathogenesis of NPC that might provide insight into this tumor's biology and could potentially be used as biomarkers. To identify these genes, we studied the epigenetics of NPC by characterizing a panel of methylation markers. Eighteen genes were evaluated by quantitative methylation\u2010specific polymerase chain reaction (PCR) in cell lines as well as in tissue samples including 50 NPC tumors and 28 benign nasopharyngeal biopsies. Significance was evaluated using Fisher's exact test and quantitative values were optimized using cut off values derived from receiver\u2013operator characteristic curves. The methylation status of <jats:italic>AIM1<\/jats:italic>, <jats:italic>APC<\/jats:italic>, <jats:italic>CALCA<\/jats:italic>, deleted in colorectal carcinomas (<jats:italic>DCC<\/jats:italic>), <jats:italic>DLEC<\/jats:italic>, deleted in liver cancer 1 (<jats:italic>DLC1<\/jats:italic>), estrogen receptor alpha (<jats:italic>ESR<\/jats:italic>), <jats:italic>FHIT<\/jats:italic>, <jats:italic>KIF1A<\/jats:italic> and <jats:italic>PGP9.5<\/jats:italic> was significantly associated with NPC compared to controls. The sensitivity of the individual genes ranged from 26 to 66% and the specificity was above 92% for all genes except FHIT. The combination of <jats:italic>PGP9.5<\/jats:italic>, <jats:italic>KIF1A<\/jats:italic> and <jats:italic>DLEC<\/jats:italic> had a sensitivity of 84% and a specificity of 92%. Ectopic expression of <jats:italic>DCC<\/jats:italic> and <jats:italic>DLC1<\/jats:italic> lead to decrease in colony formation and invasion properties. Our results indicate that methylation of novel biomarkers in NPC could be used to enhance early detection approaches. Additionally, our functional studies reveal previously unknown tumor suppressor roles in NPC.<\/jats:p>","DOI":"10.1002\/ijc.25443","type":"journal-article","created":{"date-parts":[[2010,5,6]],"date-time":"2010-05-06T20:10:30Z","timestamp":1273176630000},"page":"1393-1403","source":"Crossref","is-referenced-by-count":52,"title":["A survey of methylated candidate tumor suppressor genes in nasopharyngeal carcinoma"],"prefix":"10.1002","volume":"128","author":[{"given":"Myriam","family":"Loyo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana","family":"Brait","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Myoung S.","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kimberly L.","family":"Ostrow","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunfa 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