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NF\u03baB\u2010dependent genes are involved in antiapoptosis, cell proliferation and metastasis and are responsible for survival and proliferation of tumors. However, the mechanisms of NF\u03baB activity in CLL still need to be elucidated. Previously, we identified translocations in a region on chromosome 6q that encodes tumor necrosis factor alpha\u2010induced protein 3, which is a key player in negative feedback loop regulation of NF\u03baB. Inactivation of this ubiquitin\u2010editing enzyme is involved in immunopathologies and in tumorigenesis. Frequent mutations in the <jats:italic>A20<\/jats:italic> locus\u2014leading to sustained NF\u03baB activity\u2014could be shown to play a dominant role in development of different B\u2010cell malignancies. To check if A20 is involved in upregulation of NF\u03baB activity in CLL, we sequenced Exons 2\u20139 of the <jats:italic>A20<\/jats:italic> gene in 55 CLL DNA samples. Furthermore, we determined the methylation status of the promoter region in 63 CLL DNA samples and compared to 10 control DNAs of B cells from healthy donors. Contrary to reports from other B\u2010cell malignancies, the <jats:italic>A20<\/jats:italic> region showed neither mutations nor aberrant DNA methylation. Moreover, its expression could be confirmed by immunoblotting and showing comparable results to healthy B cells. These results indicate that malignant development in CLL differs from most of other B\u2010cell malignancies, which show frequent inactivation of A20.<\/jats:p>","DOI":"10.1002\/ijc.25579","type":"journal-article","created":{"date-parts":[[2010,7,28]],"date-time":"2010-07-28T13:45:37Z","timestamp":1280324737000},"page":"2495-2500","source":"Crossref","is-referenced-by-count":28,"title":["Sustained NF\u2010kappaB activity in chronic lymphocytic leukemia is independent of genetic and epigenetic alterations in the TNFAIP3 (<i>A20<\/i>) locus"],"prefix":"10.1002","volume":"128","author":[{"given":"Lukas 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