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Our hypothesis is that <jats:italic>Kif18A<\/jats:italic> has a role in the progression of colorectal cancer (CRC). To investigate this expression of <jats:italic>Kif18A<\/jats:italic>, mRNA was assessed by quantitative real\u2010time PCR in 113 operative specimens of primary CRC. <jats:italic>Kif18A<\/jats:italic> was overexpressed and significantly (<jats:italic>p<\/jats:italic> &lt; 0.0001) higher in CRC than in normal colon tissue. <jats:italic>Kif18A<\/jats:italic> overexpression in CRC significantly correlated with clinicopathologic factors such as tumor stage (<jats:italic>p<\/jats:italic> &lt; 0.0001), lymphatic invasion (<jats:italic>p<\/jats:italic> = 0.001), lymph node metastasis (<jats:italic>p<\/jats:italic> = 0.01), venous invasion (<jats:italic>p<\/jats:italic> = 0.002) and peritoneal dissemination (<jats:italic>p<\/jats:italic> = 0.02), suggesting that it has a key role in CRC progression. In multivariate analysis, high <jats:italic>Kif18A<\/jats:italic> expression had independent significance for poorer overall survival after resection of CRC (<jats:italic>p<\/jats:italic> = 0.037). To demonstrate <jats:italic>Kif18A<\/jats:italic>'s role in CRC progression, we performed translational and <jats:italic>in situ<\/jats:italic> studies. Using <jats:italic>in vitro<\/jats:italic> studies on CRC cell lines, we evaluated Kif18A's role in proliferation, migration and invasion. CRC cells transfected with <jats:italic>Kif18A<\/jats:italic> cDNA demonstrated significant enhanced migration (<jats:italic>p<\/jats:italic> &lt; 0.01) and invasion (<jats:italic>p<\/jats:italic> = 0.018) compared to mock\u2010transfected cells. When <jats:italic>Kif18A<\/jats:italic> was targeted with specific small interfering RNA, CRC cells had significantly reduced proliferation (<jats:italic>p<\/jats:italic> &lt; 0.01), migration (<jats:italic>p<\/jats:italic> &lt; 0.01) and invasion (<jats:italic>p<\/jats:italic> &lt; 0.05). The <jats:italic>in vitro<\/jats:italic> and translational studies demonstrated that Kif18A expression is related to events of metastasis and is a significant factor for CRC progression.<\/jats:p>","DOI":"10.1002\/ijc.25916","type":"journal-article","created":{"date-parts":[[2011,1,6]],"date-time":"2011-01-06T22:01:18Z","timestamp":1294351278000},"page":"2543-2552","source":"Crossref","is-referenced-by-count":68,"title":["Kinesin 18A expression: Clinical relevance to colorectal cancer progression"],"prefix":"10.1002","volume":"129","author":[{"given":"Makoto","family":"Nagahara","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naohiro","family":"Nishida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaaki","family":"Iwatsuki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinya","family":"Ishimaru","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koshi","family":"Mimori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumiaki","family":"Tanaka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tsuyoshi","family":"Nakagawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takanobu","family":"Sato","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kenichi","family":"Sugihara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dave S.B.","family":"Hoon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaki","family":"Mori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2011,6,9]]},"reference":[{"key":"e_1_2_6_2_2","unstructured":"World Health Organization Cancer. 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