{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,24]],"date-time":"2026-02-24T11:41:27Z","timestamp":1771933287518,"version":"3.50.1"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2011,8,30]],"date-time":"2011-08-30T00:00:00Z","timestamp":1314662400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2012,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Bmi\u20101 is overexpressed in uterine cervical cancer (UCC) and is found to be associated with adverse clinical characteristics and poor prognosis. However, little information is available on the status of circulating Bmi\u20101 mRNA in UCC. Because circulating cell\u2010free nucleic acids have emerged as a novel class of markers for cancer detection, our research aims to address this question by detecting the circulating Bmi\u20101 mRNA and to assess its diagnostic and prognostic potential in UCC. Reverse transcription quantitative real\u2010time PCR method was established to detect the circulating Bmi\u20101 mRNA in plasma of 109 patients with UCC, 138 patients with cervical intraepithelial neoplasia (CIN) and 80 healthy volunteers, and found that it was significantly increased in UCC compared with CINs and healthy controls (all at <jats:italic>p<\/jats:italic> &lt; 0.001). Moreover, its high level was significantly correlated with advanced clinical stage (<jats:italic>p<\/jats:italic> &lt; 0.001) and positive lymph nodes metastasis (<jats:italic>p<\/jats:italic> = 0.002). The area under the receiver operating characteristic curve (AUC) was 0.881, and the optimal cut\u2010off value was 0.057, providing a sensitivity of 69.7% and a specificity of 95.9%. The AUC for circulating Bmi\u20101 mRNA showed higher diagnosis capability than that for SCC\u2010Ag (<jats:italic>p<\/jats:italic> = 0.035) or CA125 (<jats:italic>p<\/jats:italic> &lt; 0.001) currently utilized. Kaplan\u2013Meier analysis demonstrated a correlation between increased circulating Bmi\u20101 mRNA level and reduced disease\u2010free survival (DFS) (<jats:italic>p<\/jats:italic> = 0.001) and overall survival (OS) (<jats:italic>p<\/jats:italic> = 0.015). And, Cox analysis indicated that it was an independent prognostic factor for DFS and OS. We conclude that circulating Bmi\u20101 mRNA may be a potential noninvasive molecular marker for diagnosis and prognosis of UCC.<\/jats:p>","DOI":"10.1002\/ijc.26360","type":"journal-article","created":{"date-parts":[[2011,8,19]],"date-time":"2011-08-19T19:29:13Z","timestamp":1313782153000},"page":"165-172","source":"Crossref","is-referenced-by-count":32,"title":["Detection of circulating Bmi\u20101 mRNA in plasma and its potential diagnostic and prognostic value for uterine cervical 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