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In the current study, ovarian carcinoma cell lines (HO\u20108910 and Ovcar\u20103) were transfected with HLA\u2010G gene. HLA\u2010G expression was analyzed with western blot and flow cytometry. Transwell experiment was performed to analyze the cell migration and invasion capability and\/or multicellular spheroid formation was investigated with the 3D culture assay <jats:italic>in vitro<\/jats:italic>. The effects of HLA\u2010G expression for tumor cell organ metastasis and for mouse survival was analyzed with the Balb\/c nu\/nu mouse model. Our data showed that HO\u20108910\u2010G and Ovcar\u20103\u2010G cells are of higher invasion potential compared with the parental HO\u20108910 and Ovcar\u20103 cells. Multicellular spheroid formation exists only in HO\u20108910\u2010G cells in a 3D culture assay. In Balb\/c nu\/nu mouse model, widespread metastasis was observed in mice xenografted with HO\u20108910\u2010G cells, but not in the group with parental cells. Mouse survival was dramatically decreased in HO\u20108910\u2010G and Ovcar\u20103\u2010G xenografted mice than that with HO\u20108910 and Ovcar\u20103 cells, respectively. In summary, our study provided the first evidence that HLA\u2010G expression is associated with tumor metastasis and with poor survival in an animal model with ovarian cancer.<\/jats:p>","DOI":"10.1002\/ijc.26375","type":"journal-article","created":{"date-parts":[[2011,8,19]],"date-time":"2011-08-19T19:33:24Z","timestamp":1313782404000},"page":"150-157","source":"Crossref","is-referenced-by-count":39,"title":["HLA\u2010G expression is associated with metastasis and poor survival in the Balb\/c nu\/nu murine tumor model with ovarian 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