{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,26]],"date-time":"2026-02-26T21:40:10Z","timestamp":1772142010371,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2011,11,8]],"date-time":"2011-11-08T00:00:00Z","timestamp":1320710400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2012,8,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Chronic opisthorchiasis caused by <jats:italic>Opisthorchis viverrini<\/jats:italic> infection is characterized by advanced periductal fibrosis leading to hepatobiliary diseases (HBD), including cholangiocarcinoma (CCA). We aimed to determine fibrotic markers to differentiate HBD status including opisthorchiasis, benign biliary disease (BBD) and CCA. Candidate fibrotic markers in plasma of healthy individuals (<jats:italic>n<\/jats:italic> = 14) and patients with opisthorchiasis (<jats:italic>n<\/jats:italic> = 32, pre\u2010 and post\u2010treatment with praziquantel), BBD (<jats:italic>n<\/jats:italic> = 31), CCA (<jats:italic>n<\/jats:italic> = 37) and other types of tumors (<jats:italic>n<\/jats:italic> = 14) were measured by ELISA and zymography. Plasma levels of hydroxyproline (HYP), collagen I, MMP\u20107 and TIMP2 in opisthorchiasis patients were significantly higher than those in healthy individuals, and MMP9\/TIMP2 balance may be associated with tissue resorption after praziquantel treatment. HYP and TIMP\u20102 levels were significantly correlated with periductal fibrosis status evaluated by ultrasonography. Plasma HYP level of CCA patients was the highest among HBD patients (<jats:italic>p<\/jats:italic> &lt; 0.05). ROC curves revealed HYP, MMP\u20107 and collagen I levels significantly distinguished opisthorchiasis, BBD and CCA (<jats:italic>p<\/jats:italic> &lt; 0.001). Odd ratio (OR) analysis demonstrated these markers in opisthorchiasis were predictable for BBD risk (<jats:italic>p<\/jats:italic> &lt; 0.05; OR = 28.50, 10.12 and 4.63 for collagen I, MMP\u20107 and HYP, respectively), and the risk was reduced by praziquantel treatment. Interestingly, only plasma HYP level in BBD was predictable for CCA risk (OR = 3.69; <jats:italic>p<\/jats:italic> = 0.020). In conclusion, plasma HYP, collagen I and MMP\u20107 may be useful as novel predictive markers of opisthorchiasis\u2010related BBD, and HYP may be used as a diagnostic marker for CCA.<\/jats:p>","DOI":"10.1002\/ijc.26443","type":"journal-article","created":{"date-parts":[[2011,9,20]],"date-time":"2011-09-20T17:24:20Z","timestamp":1316539460000},"source":"Crossref","is-referenced-by-count":19,"title":["Plasma hydroxyproline, MMP\u20107 and collagen I as novel predictive risk markers of hepatobiliary disease\u2010associated 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