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PCR\u2010based measurements of these repetitive DNA elements can be used as a surrogate marker of the genomewide methylation content. Our study aimed to identify the timing of <jats:italic>ALU<\/jats:italic> and <jats:italic>LINE\u20101<\/jats:italic> hypomethylations during multistep gastric carcinogenesis and their prognostic implications in gastric cancer (GC). In our study, we analyzed the methylation statuses of <jats:italic>ALU<\/jats:italic> and <jats:italic>LINE\u20101<\/jats:italic> in 249 cases of gastric biopsy samples and another independent set of 198 cases of advanced GC by pyrosequencing. Regardless of the <jats:italic>Helicobacter pylori<\/jats:italic> infection status, a significant decrease in the <jats:italic>ALU<\/jats:italic> methylation levels was noted during the transitions from chronic gastritis to intestinal metaplasia and from gastric adenoma to GC. <jats:italic>LINE\u20101<\/jats:italic> methylation decreased during the transition from intestinal metaplasia to gastric adenoma and no further decrease occurred during the transition from gastric adenoma to GC. A low <jats:italic>LINE\u20101<\/jats:italic> methylation status was strongly associated with poor prognosis in GC. A multivariate analysis revealed that <jats:italic>LINE\u20101<\/jats:italic> methylation status was an independent prognostic factor. Our findings suggest that <jats:italic>ALU<\/jats:italic> and <jats:italic>LINE\u20101<\/jats:italic> hypomethylations are early events during multistep gastric carcinogenesis. Furthermore, the <jats:italic>LINE\u20101<\/jats:italic> methylation status can be used as a molecular biomarker to define a subset of GC patients with poor prognosis.<\/jats:p>","DOI":"10.1002\/ijc.27369","type":"journal-article","created":{"date-parts":[[2011,11,28]],"date-time":"2011-11-28T10:06:01Z","timestamp":1322474761000},"page":"1323-1331","source":"Crossref","is-referenced-by-count":72,"title":["<i>ALU<\/i> and <i>LINE\u20101<\/i> hypomethylations in multistep gastric carcinogenesis and their prognostic implications"],"prefix":"10.1002","volume":"131","author":[{"given":"Jeong 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