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The WASF3 promoter region contains HIF1A response elements (HRE). ChIP assays demonstrate that HIF1A binds to these HRE elements in the promoter region, and luciferase reporter assays using the WASF3 gene minimal promoter shows that hypoxia results in its upregulation. Phosphorylation of WASF3 is required for its ability to affect invasion and increased phosphoactivation of WASF3 is also seen in cells challenged with hypoxia. These cells also show increased motility in the scratch wound assay. Cells in which WASF3 has been knocked down show no response to hypoxia as expected, implicating the specificity of the hypoxic response to WASF3. Overall, these experiments demonstrate WASF3 is a HIF1A\u2010regulated gene and suggests a mechanism to explain the observation of elevated expression of WASF3 in advanced stage tumors.<\/jats:p>","DOI":"10.1002\/ijc.27631","type":"journal-article","created":{"date-parts":[[2012,5,14]],"date-time":"2012-05-14T10:06:44Z","timestamp":1336990004000},"source":"Crossref","is-referenced-by-count":32,"title":["HIF1A induces expression of the WASF3 metastasis\u2010associated gene under hypoxic conditions"],"prefix":"10.1002","volume":"131","author":[{"given":"Pushpankur","family":"Ghoshal","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong","family":"Teng","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leslie Ann","family":"Lesoon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John 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