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We demonstrated earlier that a mixture of experimentally selected substances (\u201cactive mixture\u201d, AM: <jats:sc>L<\/jats:sc>\u2010arginine, <jats:sc>L<\/jats:sc>\u2010histidine, <jats:sc>L<\/jats:sc>\u2010methionine, <jats:sc>L<\/jats:sc>\u2010phenylalanine, <jats:sc>L<\/jats:sc>\u2010tyrosine, <jats:sc>L<\/jats:sc>\u2010tryptophan, <jats:sc>L<\/jats:sc>\u2010ascorbate, <jats:sc> D<\/jats:sc>\u2010biotin, pyridoxine, riboflavin, adenine, <jats:sc>L<\/jats:sc>(\u2010)malate) possesses a selective toxic effect <jats:italic>in vitro<\/jats:italic> on a variety of tumor cell lines, and we have shown that the AM selectively induces apoptosis of cancer cells <jats:italic>in vitro<\/jats:italic>. To explore the <jats:italic>in vivo<\/jats:italic> significance of our earlier findings we examined the antitumor effect of AM in Colon 26 murine colorectal adenocarcinoma, B16 murine melanoma, MXT murine mammary carcinoma, S180 murine sarcoma, P388 murine lymphoid leukemia, HL\u201060 human promyeloid leukemia, PC\u20103 human prostate carcinoma, and HT\u201029 human colon carcinoma tumor models. Treatment of tumor bearing mice with AM inhibited the growth of the tumors investigated, with an inhibitory effect ranging from 40 to 69%. The AM had a comparable antitumor effect with 5\u2010fluorouracil and cisplatin in the Colon\u201026 tumor model, and combined treatment with AM and 5\u2010fluorouracil or cisplatin resulted in an enhanced tumor growth inhibitory effect. The AM induced apoptosis through the mitochondrial pathway and induced G1 arrest in PC\u20103 cells and increased the number of apoptotic cells in PC\u20103 xenografts. These findings suggest that the AM might offer an interesting perspective in the treatment of cancer and in combination with other treatments may offer hope for a more effective cancer therapy.<\/jats:p>","DOI":"10.1002\/ijc.27756","type":"journal-article","created":{"date-parts":[[2012,8,2]],"date-time":"2012-08-02T12:36:38Z","timestamp":1343910998000},"page":"1213-1221","source":"Crossref","is-referenced-by-count":10,"title":["A mixture of amino acids and other small molecules present in the serum suppresses the growth of murine and human tumors <i>in vivo<\/i>"],"prefix":"10.1002","volume":"132","author":[{"given":"Gyula","family":"Kulcs\u00e1r","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dezs\u0151","family":"Ga\u00e1l","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P\u00e9ter 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