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In this study, we evaluated the therapeutic efficacy of a novel survivin\u2010based vaccine by subcutaneous or intraperitoneum injection of BALB\/c mice with murine fiber\u2010induced MM tumor cells followed by vaccination with recombinant Fowlpox virus replicons encoding survivin. Vaccination generated significant immune responses in both models, leading to delayed tumor growth and improved animal survival. Flow cytometry and immunofluorescence analyses of tumors from vaccinated mice showed CD8<jats:sup>+<\/jats:sup> T\u2010cell infiltration, and real\u2010time PCR demonstrated increased mRNA and protein levels of immunostimulatory cytokines. Analyses of survivin peptide\u2010pulsed spleen and lymph node cells from vaccinated mice using ELISPOT and intracellular cytokine staining confirmed antigen\u2010specific, interferon\u2010\u03b3\u2010producing CD8<jats:sup>+<\/jats:sup> T\u2010cell responses. In addition pentamer\u2010based flow cytometry showed that vaccination generated survivin\u2010specific CD8<jats:sup>+<\/jats:sup> T cells. Importantly, vaccination did not affect fertility or induce autoimmune abnormalities in mice. Our results demonstrate that vaccination with recombinant Fowlpox expressing survivin improves T\u2010cell responses against aggressive MM tumors and may form the basis for promising clinical applications.<\/jats:p>","DOI":"10.1002\/ijc.28048","type":"journal-article","created":{"date-parts":[[2013,1,21]],"date-time":"2013-01-21T09:58:47Z","timestamp":1358762327000},"page":"612-623","source":"Crossref","is-referenced-by-count":15,"title":["Fowlpox\u2010based survivin vaccination for malignant mesothelioma therapy"],"prefix":"10.1002","volume":"133","author":[{"given":"Pietro","family":"Bertino","sequence":"first","affiliation":[{"name":"Department of Cell and Molecular Biology John A. 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