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Various plant triterpenoids and their synthetic analogs have shown significant promise as potent chemopreventive agents in breast cancer. The current study was initiated to investigate mechanism\u2010based chemopreventive potential of a novel synthetic oleanane triterpenoid (methyl\u201025\u2010hydroxy\u20103\u2010oxoolean\u201012\u2010en\u201028\u2010oate, AMR\u2010Me) against 7,12\u2010dimethylbenz(<jats:italic>a<\/jats:italic>)anthracene (DMBA)\u2010initiated rat mammary carcinogenesis, an experimental rodent tumor model that closely resembles human mammary cancer. Rats were orally administered with AMR\u2010Me (0.8, 1.2 and 1.6 mg\/kg) three times per week for 18 weeks. Following two weeks of AMR\u2010Me treatment, mammary carcinogenesis was initiated by oral administration of DMBA (50 mg\/kg body weight). At the end of the study (16 weeks following DMBA exposure), AMR\u2010Me exhibited a striking inhibition of DMBA\u2010induced mammary tumor incidence, total tumor burden, average tumor weight and reversed histopathological alterations without toxicity. AMR\u2010Me dose\u2010dependently suppressed abnormal cell proliferation, induced apoptosis, up\u2010regulated pro\u2010apoptotic protein Bax and down\u2010regulated antiapoptotic protein Bcl\u20102 in mammary tumors. AMR\u2010Me upregulated the transcriptional levels of Bax, Bad, caspase\u20103, caspase\u20107 and poly(ADP\u2010ribose) polymerase and down\u2010regulated Bcl\u20102. These results clearly demonstrate for the first time that novel triterpenoid AMR\u2010Me exerts chemopreventive efficacy in the classical DMBA model of breast cancer by suppressing abnormal cell proliferation and inducing apoptosis mediated through mitochondrial pro\u2010apoptotic mechanisms. AMR\u2010Me could be developed as a chemopreventive drug to reduce the risk of human breast cancer that remains a devastating disease.<\/jats:p>","DOI":"10.1002\/ijc.28108","type":"journal-article","created":{"date-parts":[[2013,2,13]],"date-time":"2013-02-13T04:05:15Z","timestamp":1360728315000},"page":"1054-1063","source":"Crossref","is-referenced-by-count":38,"title":["Chemopreventive effect of a novel oleanane triterpenoid in a chemically induced rodent model of breast cancer"],"prefix":"10.1002","volume":"133","author":[{"given":"Anupam","family":"Bishayee","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Sciences School of Pharmacy, American University of Health Sciences Signal Hill CA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Animesh","family":"Mandal","sequence":"additional","affiliation":[{"name":"Cancer Therapeutics and Chemoprevention Group Department of Pharmaceutical Sciences College of Pharmacy, Northeast Ohio Medical 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