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We evaluated the validity and efficacy of the SDB method for the diagnosis of lymph node metastasis in a clinical setting (Trial 1). To evaluate the validity of the SDB method in clinical specimens, 180 dissected lymph nodes from 29 cases, including breast, gastric and colorectal cancer, were examined. Each lymph node was sliced at the maximum diameter and the sensitivity, specificity and accuracy of the SDB method were determined and compared with the final pathology report. Metastasis was detected in 32 lymph nodes (17.8%), and the sensitivity, specificity and accuracy of the SDB method were 100, 98.0 and 98.3%, respectively (Trial 2). To evaluate the efficacy of the SDB method in sentinel lymph node (SLN) biopsy, 174 SLNs from 100 cases of clinically node\u2010negative breast cancer were analyzed. Each SLN was longitudinally sliced at 2\u2010mm intervals and the sensitivity, specificity, accuracy and time required for the SDB method were determined and compared with the intraoperative pathology report. Metastasis was detected in 15 SLNs (8.6%), and the sensitivity, specificity, accuracy and mean required time of the SDB method were 93.3, 96.9, 96.6 and 43.3 min, respectively. The SDB method is a novel and reliable modality for the intraoperative diagnosis of SLN metastasis.<\/jats:p>","DOI":"10.1002\/ijc.28408","type":"journal-article","created":{"date-parts":[[2013,8,2]],"date-time":"2013-08-02T09:44:37Z","timestamp":1375436677000},"page":"905-912","source":"Crossref","is-referenced-by-count":8,"title":["Novel diagnostic procedure for determining metastasis to sentinel lymph nodes in breast cancer using a semi\u2010dry dot\u2010blot method"],"prefix":"10.1002","volume":"134","author":[{"given":"Ryota","family":"Otsubo","sequence":"first","affiliation":[{"name":"Department of Surgical Oncology Nagasaki University Hospital Nagasaki Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masahiro","family":"Oikawa","sequence":"additional","affiliation":[{"name":"Department of Surgical Oncology Nagasaki University Hospital Nagasaki 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