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HSP27 is increased in many cancers at advanced stage including ovarian cancer and associated with cancer resistance to therapy and poor patients' survival. The phosphorylation of HSP27 regulates both its chaperone activity and its control of cytoskeletal stability. We show that HSP27 was necessary for the remodeling of actin filaments induced by HGF and that motility <jats:italic>in vitro<\/jats:italic> depended on the p38MAPK\u2010MK2 axis. <jats:italic>In vivo<\/jats:italic>, HSP27 silencing impaired the ability of the highly metastatic, HGF\u2010secreting ovarian cancer cells to give rise to spontaneous metastases. This was due to defective motility across the vessel wall and reduced growth. Indeed, HSP27 silencing impaired the ability of circulating ovarian cancer cells to home to the lungs and to form experimental hematogenous metastases and the capability of cancer cells to grow as subcutaneous xenografts. Moreover, HSP27 suppression resulted in the sensitization of xenografts to low doses of the chemotherapeutic paclitaxel, likely because HSP27 protected microtubules from bundling caused by the drug. Altogether, these data show that the HSP27 is required for the proinvasive and prometastatic activity of HGF and suggest that HSP27 might be not only a marker of progression of ovarian cancer, but also a suitable target for therapy.<\/jats:p>","DOI":"10.1002\/ijc.28464","type":"journal-article","created":{"date-parts":[[2013,8,28]],"date-time":"2013-08-28T13:02:09Z","timestamp":1377694929000},"page":"1289-1299","source":"Crossref","is-referenced-by-count":45,"title":["HSP27 is required for invasion and metastasis triggered by hepatocyte growth factor"],"prefix":"10.1002","volume":"134","author":[{"given":"Simona","family":"Pavan","sequence":"first","affiliation":[{"name":"Department of Oncology University of Torino, School of Medicine Torino Italy"},{"name":"Laboratory of Cancer Genetics Institute for Cancer Research at Candiolo Torino 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