{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T15:57:00Z","timestamp":1789055820440,"version":"build-2803163510"},"reference-count":46,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2013,10,15]],"date-time":"2013-10-15T00:00:00Z","timestamp":1381795200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:p>Women with breast cancer (BC) and antithyroid peroxidase (TPO) autoantibodies (TPOAb) have a better prognosis than women lacking TPOAb. Sera from women with TPOAb displayed immunoreactivity to BC tissue by immunofluorescence that was not apparent in women without TPOAb. We hypothesize a BC\/thyroid shared antigen that provides a target for humoral or cell\u2010mediated immune activity; candidates include the sodium\/iodide symporter (expressed in thyroid and BC), cross\u2010reacting epitopes in TPO and lactoperoxidase (LPO) or TPO itself. As the association is with TPOAb, we investigated TPO expression in BC, breast peritumoral tissue (PT), other tissues (tumoral and not) and thyroid as positive control. Transcripts for known and novel TPO isoforms were detected in BC (<jats:italic>n<\/jats:italic>\u2009=\u20098) and PT (<jats:italic>n<\/jats:italic>\u2009=\u20098) but at approximately 10<jats:sup>4<\/jats:sup>\u2010fold lower than in thyroid while in non\u2010BC tumors (<jats:italic>n<\/jats:italic>\u2009=\u20095) they were at the limit of detection. TPO was expressed also in adipose tissue (<jats:italic>n<\/jats:italic>\u2009=\u200917), 10<jats:sup>3<\/jats:sup>\u2010fold lower than in thyroid. Full length TPO (Mr 105\u2013110 kDa) was detected in Western blots in the majority of examined tissues; preabsorption of the TPO antibody with recombinant TPO (but not LPO) reduced the signal, indicating specificity. The same occurred with some lower molecular weight bands, which could correspond to smaller TPO transcript isoforms, present in all samples. In conclusion, TPO is weakly expressed in BC and other tissues; this could partly explain the high frequency and protective role of TPOAb in BC patients. Further studies will investigate tissue specificity, function and immunogenicity of the novel TPO variants (some BC\u2010specific) identified.<\/jats:p>","DOI":"10.1002\/ijc.28493","type":"journal-article","created":{"date-parts":[[2013,10,12]],"date-time":"2013-10-12T01:45:03Z","timestamp":1381542303000},"page":"1706-1714","source":"Crossref","is-referenced-by-count":38,"title":["Does thyroid peroxidase provide an antigenic link between thyroid autoimmunity and breast cancer?"],"prefix":"10.1002","volume":"134","author":[{"given":"I.","family":"Muller","sequence":"first","affiliation":[{"name":"Thyroid Research Group Institute of Molecular &amp; Experimental Medicine School of Medicine Cardiff University Cardiff Wales United Kingdom"},{"name":"Department of Endocrinology University of Pisa Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C.","family":"Giani","sequence":"additional","affiliation":[{"name":"Department of Endocrinology University of Pisa Pisa Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"L.","family":"Zhang","sequence":"additional","affiliation":[{"name":"Thyroid Research Group Institute of Molecular &amp; 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