{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,19]],"date-time":"2025-11-19T06:54:28Z","timestamp":1763535268449},"reference-count":30,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2014,4,29]],"date-time":"2014-04-29T00:00:00Z","timestamp":1398729600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"the Helmholtz Alliance on Immunotherapy of Cancer"},{"name":"INNOCHEM","award":["LSHB-CT-2005-518167"],"award-info":[{"award-number":["LSHB-CT-2005-518167"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[2014,12]]},"abstract":"<jats:p>Bispecific antibodies are promising agents for immunotherapy. Here, we describe a quadroma\u2010based trifunctional bispecific antibody binding the chemokine receptor CXCR5 and the T\u2010cell antigen CD3 that efficiently prevents tumor growth in a mouse B\u2010cell lymphoma model. CXCR5 regulates the tissue homeostasis of mature B cells and is highly expressed on B\u2010cell non\u2010Hodgkin and lymphocyte\u2010predominant Hodgkin lymphoma, as well as on a subset of CD4<jats:sup>+<\/jats:sup> T cells known as follicular T\u2010helper cells. <jats:italic>In vitro<\/jats:italic>, the bispecific CXCR5::CD3 antibody efficiently recruited effector T cells to CXCR5 expressing B cells and induced a co\u2010stimulation\u2010independent activation of CD8<jats:sup>+<\/jats:sup> and CD4<jats:sup>+<\/jats:sup> T cells as demonstrated by the <jats:italic>de novo<\/jats:italic> expression of CD25 and CD69, and secretion of the cytokines IFN\u2010\u03b3, TNF\u2010\u03b1, IL\u20106 and IL\u201010 by peripheral blood mononuclear cells. Notably, at low antibody concentrations, CXCR5::CD3 displayed a significantly higher cytotoxic activity against autologous B cells than its parental antibodies or rituximab. <jats:italic>In vivo<\/jats:italic> imaging revealed that CXCR5::CD3 and its parental CXCR5 antibody efficiently prevent tumor growth in a xenograft model of B\u2010cell lymphoma in mice and prolong their survival. Taken together, our results identify CXCR5 as a promising target for antibody\u2010based therapies in the treatment of B\u2010cell malignancies.<\/jats:p>","DOI":"10.1002\/ijc.28893","type":"journal-article","created":{"date-parts":[[2014,4,12]],"date-time":"2014-04-12T08:21:03Z","timestamp":1397290863000},"page":"2623-2632","source":"Crossref","is-referenced-by-count":18,"title":["Immunotherapy of B\u2010cell non\u2010Hodgkin lymphoma by targeting the chemokine receptor CXCR5 in a preclinical mouse model"],"prefix":"10.1002","volume":"135","author":[{"given":"Hossein","family":"Panjideh","sequence":"first","affiliation":[{"name":"Department of Tumor Genetics and Immunogenetics Max\u2010Delbr\u00fcck\u2010Center of Molecular Medicine, MDC Robert\u2010Roessle\u2010Stra\u00dfe 10 Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gerd","family":"M\u00fcller","sequence":"additional","affiliation":[{"name":"Department of Tumor Genetics and Immunogenetics 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