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The ACIF procedure, in which the cell smears were thoroughly air\u2010dried and fixed with carbon tetrachloride (for 15 min at room temperature), provided reproducible staining results with minimum variations. The fixed smears could be stored for at least 5 weeks at \u22128\u00b0 C without loss of reactivity. On the basis of the above evaluation, a total of 59 human cell lines, which had unknown or uncertain association with EBV, were examined for the presence or absence of EBNA by the ACIF procedure. All of the cell lines tested could be judged positive or negative for EBNA. All of 21 lines growing as monolayer or in a mixed state of monolayer and suspension, were negative. Of 38 lines grown in suspension, 36 lymphoblastoid lines were positive but the remaining 2 lines (seemingly not lymphoblastoid) were negative.<\/jats:p>","DOI":"10.1002\/ijc.2910140609","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T15:10:09Z","timestamp":1171897809000},"page":"753-761","source":"Crossref","is-referenced-by-count":29,"title":["Evaluation of epstein\u2010barr virus\u2010associated nuclear antigen with various human cell lines"],"prefix":"10.1002","volume":"14","author":[{"given":"Masahiro","family":"Suzuki","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yorio","family":"Hinuma","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,18]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"309","article-title":"Chromosomal aberrations of a lymphoblastoid cell line by experimental infection with Epstein\u2010Barr virus","volume":"64","author":"Amano K.","year":"1973","journal-title":"Gann"},{"key":"e_1_2_1_3_1","first-page":"549","article-title":"Establishment of a new human cell line derived from non\u2010malignant endometrium","volume":"62","author":"Arata T.","year":"1971","journal-title":"Gann"},{"key":"e_1_2_1_4_1","first-page":"649","article-title":"Transplantation of a newly established human cancer cell line","volume":"60","author":"Arata T.","year":"1969","journal-title":"Gann"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"1257","DOI":"10.1128\/jb.91.3.1257-1262.1966","article-title":"Complement\u2010fixation test with cell lines derived from Burkitt's lymphoma and acute leukemias","volume":"91","author":"Armstrong D.","year":"1966","journal-title":"J. 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