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Sera from 33 patients with other tumors, 7 patients with non\u2010neoplastic diseases and 50 healthy individuals did not show any IA reactivity towards melanoma cells. The reaction seemed to be selectively directed against tumor\u2010associated antigens (TAA) on melanoma cells. No correlation with the stage of the disease could be found. Longitudinal studies indicated that conversions in antibody activity did not correlate with the clinical state of the patients. There was also no correlation with the corresponding in vitro data obtained in cell\u2010mediated immunity tests. Cell lines and short\u2010term cultures originating from tumors from different melanoma patients shared a common antigenicity. The expression of TAA on cells from a melanoma cell line fluctuated significantly during prolonged culture. The expression of TAA was influenced by the culture conditions and the growth state of the cells. A relation between TAA\u2010expression and cell cycle phase was demonstrated.<\/jats:p>","DOI":"10.1002\/ijc.2910160612","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T16:08:03Z","timestamp":1171901283000},"page":"981-997","source":"Crossref","is-referenced-by-count":34,"title":["Antibodies and antigen expression in human melanoma detected by the immune adherence test"],"prefix":"10.1002","volume":"16","author":[{"given":"Santoso","family":"Cornain","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan E.","family":"De Vries","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Collard","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claudia","family":"Vennegoor","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ineke","family":"Van Wingerden","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philip","family":"R\u00fcmke","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/newbio238185a0"},{"key":"e_1_2_1_3_1","first-page":"37","article-title":"Significance of serum factors modifying cellular immune responses to growing tumours","volume":"28","author":"Baldwin R. 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