{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T00:34:42Z","timestamp":1699835682834},"reference-count":35,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":10928,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1976,8,15]]},"abstract":"Abstract<\/jats:title>The infection of isolated B and T cells by a murine leukemia virus (Friend) (MuLV\u2010F) was studied both in vitro<\/jats:italic> and in vitro<\/jats:italic> with an implanted diffusion chamber system. Lymphocytes were obtained from pools of normal spleen cells by filtration of the cell suspension through a nylon\u2010wool column. The purity for both Ig positive and theta\u2010positive cells varied between 85% and 90% in the B\u2010cell and T\u2010cell fractions; both lymphocyte fractions responded very well to stimulation with their respective specific polyclonal mitogens, bacterial lipopolysaccharide (LPS) and concanavalin A (Con A). Lymphocytes were infected by incubating pelleted cells in 2\u20136 \u00d7 104<\/jats:sup> FFU MuLV for 1 h at 4\u00b0C and were then cultured for 5\u201310 days. Cells releasing infectious MuLV were enumerated as infectious centers (IC). IC were readily detectable in the cultures of infected B\u2010cells but none were found in the T\u2010cell cultures. Addition of LPS to the culture medium increased the number of IC in B\u2010cell fractions up to 1,000\u2010fold. Furthermore, in T\u2010cell cultures with LPS, IC also appeared in numbers which approximately correlated with the contaminating Ig+<\/jats:sup> cells of the T\u2010cell fraction. In contrast, Con A had no consistent effect on the infection of either B or T cells. In the absence of MuLV\u2010F, mitogenic stimulation alone did not elicit any endogenous IC. In subsequent experiments, purified lymphocytes were infected in diffusion chambers in vivo<\/jats:italic>. The number of IC in infected B<\/jats:bold> cells increased 1,000\u2010fold as compared to infection in tissue culture. The peak of infection at 10 days was followed by a slight decline. Infected cells were also found in diffusion chambers containing T\u2010cell fractions; these IC had very similar kinetics to those in B<\/jats:bold>\u2010cell\u2010containing chambers, but their number was 10 times lower, suggesting that the infected cells were B cells, which comprised about 10% of the T\u2010cell fraction. The virus\u2010related antigens were detectable by immunofluorescence on the membrane of cells recovered from B\u2010cell\u2010bearing chambers but not on cells from T\u2010cell\u2010bearing chambers.<\/jats:p>","DOI":"10.1002\/ijc.2910180208","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T15:58:50Z","timestamp":1171900730000},"page":"189-196","source":"Crossref","is-referenced-by-count":20,"title":["Interactions of murine leukemia virus (MuLV) with isolated lymphocytes. II. Infection of B and T cells with friend virus complex in diffusion chambers and in vitro<\/i>: Effect of polyclonal mitogens"],"prefix":"10.1002","volume":"18","author":[{"given":"Jan","family":"Cerny","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patricia A.","family":"Hensgen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samuel H.","family":"Fistel","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lynn Mastalir","family":"Demler","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_1_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910050114"},{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910060217"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/249063a0"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910150219"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910180207"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910180209"},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"943","DOI":"10.4049\/jimmunol.115.4.943","article-title":"Immunosuppression by spleen cells from Moloney leukemia. 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