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The level of SLMC was assessed by means of two previously established assay systems; the xenogeneic assay involving the mouse mastocytoma line P815, and the allogeneic assay in which the human chronic myelogenous leukemia\u2010derived line, K562, was used. The assay systems involve the use of Ficoll\u2010Isopaque\u2010separated, iron\u2010plus\u2010magnetism\u2010purified lymphocytes in an overnight <jats:sup>51<\/jats:sup>chromium release assay, and reflect the cytotoxic ability of human non\u2010T, complement receptor\u2010, F<jats:sub>c<\/jats:sub> receptor\u2010positive lymphocytes. In the present paper, lymphocytes from all normal donors tested showed significant activity in the SLMC assay, with some variation from day to day. This variation was markedly reduced when different normal donors were tested on the same day and under identical experimental conditions. In contrast, lymphocytes from many patients with malignant disease had decreased SLM activity, and this decrease was highly significant in patients with treated or untreated metastatic disease, or untreated CLL. This was also the case when the data were expressed relative to the number of cytotoxic cells in the normal control population, or in comparison to the relative SLMC activity of lymphocytes from patients with other conditions. Markedly decreased SLMC was observed in some patients in spite of normal T and B lymphocyte proportions, or the presence of the ability to mount a vigorous delayed hypersensitivity reaction to PPD. A comparison of the xenogeneic and allogeneic assays showed that the same information with respect to whether SLMC was normal or abnormal was obtained with both assays in the majority of cases. The significance of the data is discussed with respect to the possible role of SLMC in vivo and the relevance of SLMC to the assessment of specific cell\u2010mediated cytotoxicity in malignant disease.<\/jats:p>","DOI":"10.1002\/ijc.2910180508","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T16:31:41Z","timestamp":1171902701000},"page":"593-604","source":"Crossref","is-referenced-by-count":173,"title":["Spontaneous human lymphocyte\u2010mediated cytotoxicity againts tumour target cells. I. 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