{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,27]],"date-time":"2025-09-27T22:05:30Z","timestamp":1759010730600},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":10744,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1977,2,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>These data describe the use of a passive hemagglutination test (PHAT) for the detection of antibodies to the Epstein\u2010Barr virus (EBV) associated antigen, early antigen\u2010D (EA\u2010D). EA\u2010D was obtained from EBV\u2010superinfected RPMI 64\u201310 cell preparations which showed by immunofluorescent staining (IF) that 25\u201335% of the cells contained EA\u2010D. Solubilization of EA\u2010D was achieved by sonic oscillation, treatment of the pellet\u2010associated material with triton X 100, ammonium sulfate precipitation, and chromatography on diethylaminoethyl celluiose. Successful use of the PHAT for detection of antibodies to EA\u2010D was dependent on the degree of purity of the partially purified antigen. Throughout these studies preparations from uninfected 64\u201010 cells were used as control reagents. More than 100 human antisera from patients with EBV\u2010associated diseases, which had been previously tested by standard IF procedures were assayed by PHAT. A good correlation was found between IF and PHAT titers for EA\u2010D and, in addition, the PHAT was 50\u2013100 times more sensitive. Most human sera gave no agglutination of red cells coated with control preparations. An inhibition (blocking) of PHAT was developed which was useful in the purification and characterization of EA\u2010D. These data indicate that the PHAT may provide a rapid, reliable, objective and sensitive method for studying EA\u2010D and possibly other virus\u2010associated antigens.<\/jats:p>","DOI":"10.1002\/ijc.2910190203","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T16:25:47Z","timestamp":1171902347000},"page":"150-160","source":"Crossref","is-referenced-by-count":5,"title":["Detection of epstein\u2010barr virus early antigen\u2010d and its antibodies by passive hemagglutination"],"prefix":"10.1002","volume":"19","author":[{"given":"Clifton A.","family":"Ogburn","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbara Ann","family":"Zajac","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"1257","DOI":"10.1128\/jb.91.3.1257-1262.1966","article-title":"Complement fixation tests with cell lines derived from Burkitt's lymphoma and acute leukemias","volume":"91","author":"Armstrong D.","year":"1966","journal-title":"J. Bact."},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.93.2.107"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"751","DOI":"10.1093\/jnci\/51.3.751","article-title":"Differences in heat stability of antigens associated with Epstein\u2010Barr virus demonstrated by immunodiffusion","volume":"51","author":"Demissie A.","year":"1973","journal-title":"J. nat. Cancer Inst."},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.99.2.167"},{"key":"e_1_2_1_6_1","first-page":"133","article-title":"Studies on the relation of membrane immunofluorescence to Epstein\u2010Barr virus infection","volume":"44","author":"Dunkel V. C.","year":"1970","journal-title":"J. nat. Cancer Inst."},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910130608"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-134-34875"},{"key":"e_1_2_1_9_1","series-title":"IARC Scientific Publication No. 2","first-page":"302","volume-title":"Oncogenesis and herpes\u2010viruses","author":"Greenland T.","year":"1972"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1128\/JB.91.3.1248-1256.1966"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910080212"},{"key":"e_1_2_1_12_1","first-page":"861","article-title":"Antibodies to early Epstein\u2010Barr virus\u2010induced antigens in Burkitt's lymphoma","volume":"46","author":"Henle G.","year":"1971","journal-title":"J. nat. Cancer Inst."},{"key":"e_1_2_1_13_1","first-page":"225","article-title":"Antibodies to EB virus in nasopharyngeal carcinoma, other head and neck neoplasms and control groups","volume":"44","author":"Henle W.","year":"1970","journal-title":"J. nat. Cancer Inst."},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1093\/infdis\/124.1.58"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.169.3941.188"},{"key":"e_1_2_1_16_1","doi-asserted-by":"crossref","first-page":"949","DOI":"10.1093\/jnci\/53.4.949","article-title":"Detection of Epstein\u2010Barr virus\u2010determined intracellular antigens in unfixed cells with labeled antibodies","volume":"53","author":"Hilgers F.","year":"1974","journal-title":"J. nat. Cancer Inst."},{"key":"e_1_2_1_17_1","first-page":"2279","article-title":"Cultivation in vitro of myeloblasts from human leukemia","volume":"64","author":"Ikawata S.","year":"1964","journal-title":"N. Y. State J. Med."},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910050204"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.128.5.1011"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(196904)23:4<990::AID-CNCR2820230437>3.0.CO;2-V"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1097\/00007890-196503000-00006"},{"key":"e_1_2_1_23_1","doi-asserted-by":"crossref","first-page":"713","DOI":"10.4049\/jimmunol.99.4.713","article-title":"Papain fragments of rabbit anti\u2010leukocyte antibodies: effects in relation to some serologic and biologic activities of the antiserum","volume":"99","author":"Ogburn C. A.","year":"1967","journal-title":"J. Immunol."},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.56.6.1699"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(64)92345-1"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910150406"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910110302"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910090120"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910050210"},{"key":"e_1_2_1_30_1","first-page":"865","article-title":"Antibodies in human sera to soluble and viral antigens found in Burkitt's lymphoma and other lymphoblastoid cell lines","volume":"44","author":"Vonka V.","year":"1970","journal-title":"J. nat. Cancer Inst."},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910080105"},{"key":"e_1_2_1_32_1","series-title":"IARC Scientific Publication No. 75 003","first-page":"210","volume-title":"International workshop on EBV","author":"Zajac B. A.","year":"1975"},{"key":"e_1_2_1_33_1","series-title":"IARC Scientific Publication No. 11","first-page":"331","volume-title":"Oncogenesis and herpesviruses II","author":"Zajac B. A.","year":"1975"}],"container-title":["International Journal of Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fijc.2910190203","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijc.2910190203","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,12]],"date-time":"2023-11-12T09:02:02Z","timestamp":1699779722000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ijc.2910190203"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1977,2,15]]},"references-count":32,"journal-issue":{"issue":"2","published-print":{"date-parts":[[1977,2,15]]}},"alternative-id":["10.1002\/ijc.2910190203"],"URL":"https:\/\/doi.org\/10.1002\/ijc.2910190203","archive":["Portico"],"relation":{},"ISSN":["0020-7136","1097-0215"],"issn-type":[{"value":"0020-7136","type":"print"},{"value":"1097-0215","type":"electronic"}],"subject":[],"published":{"date-parts":[[1977,2,15]]}}}