{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,13]],"date-time":"2023-11-13T00:07:20Z","timestamp":1699834040208},"reference-count":19,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":10716,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1977,3,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Virus\u2010specific antigens were detected in Luck\u00e9 turnor cells by indirect immunofluorescence using antiserum prepared against Luck\u00e9 herpesvirus. Intracellular fluorescence, both cytoplasmic and nuclear, was observed only in acetone:methanol\u2010fixed tumor cells that contained herpesvirus detected by electron microscopy. The number of positive cells correlated well with the number of cells containing virus. In contrast, both virus\u2010containing and virus\u2010free cells exhibited membrane fluorescence when viable unfixed tumor cells were tested. A striking reduction in the number of membrane fluorescent cells was observed with an increase in the length of time that tumor cells were in primary culture. No reaction was observed with a variety of normal <jats:italic>R. pipiens<\/jats:italic> cells. Absorption of the antiserum with normal frog kidney tissue had no effect on the number of positive cells whereas absorption with virus\u2010free tumor reduced the reaction; absorption with virus\u2010containing tumor eliminated it. These findings provide the first demonstration that the Luck\u00e9 herpesvirus genome resident in virus\u2010free tumor cells expresses a virus\u2010associated membrane antigen(s).<\/jats:p>","DOI":"10.1002\/ijc.2910190320","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T16:25:43Z","timestamp":1171902343000},"page":"414-418","source":"Crossref","is-referenced-by-count":8,"title":["Viruses and renal carcinoma of <i>rana pipiens.<\/i> XV. The presence of virus\u2010associated membrane antigen(s) on luck\u00e9 tumor cells"],"prefix":"10.1002","volume":"19","author":[{"given":"R. F.","family":"Naegele","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Granoff","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0042-6822(73)81007-4"},{"key":"e_1_2_1_3_1","first-page":"810","article-title":"Herpesvirus type 2 related antigens and their relevance to humoral and cell\u2010mediated immunity in patients with cervical cancer","volume":"36","author":"Aurelian L.","year":"1976","journal-title":"Cancer Res."},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/225645a0"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.181.4098.448"},{"key":"e_1_2_1_6_1","first-page":"19","volume-title":"Methods in cell physiology","author":"Freed J. 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