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In agreement with results from other laboratories, antisera to type\u2010C and the non\u2010type\u2010C bovine leukemia and equine infectious anemia viruses did not precipitate <jats:sup>125<\/jats:sup>I\u2010labelled MPMV p25. In addition, these viruses did not cross\u2010react in a competition radioimmunoassay for MPMV p25. Twenty\u2010one human tissues (15 breast carcinomas, 2 normal breasts, 3 acute myelogenous leukemias and 1 sarcoma) were fractionated by detergent solubilization, ammonium sulfate precipitation, and DE\u201052 anion exchange chromatography. These methods were shown to be highly effective for purification of MPMV p25. Under assay conditions which minimized incubation damage to the <jats:sup>125<\/jats:sup>I\u2010MPMV p25, all tissues failed to react in the competition radioimmunoassay (RIAT). Two hundred and two human sera or plasma specimens, including those from patients with breast cancer and 33 age\u2010matched controls, from 50 patients with hematologic malignancies, from 12 patients with amyotrophic lateral sclerosis, and from 14 patients with systemic lupus erythematosis, were examined for antibodies to MPMV p25. With the exception of two multiple myeloma plasmas which produced artifactual false positive reactions based on hypergammaglobulinemia, a known complication of salt precipitation radioimmunoassays, the remainder of the specimens were negative for evidence of MPMV p25 antibodies.<\/jats:p>","DOI":"10.1002\/ijc.2910190410","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T16:25:41Z","timestamp":1171902341000},"page":"498-504","source":"Crossref","is-referenced-by-count":19,"title":["Radioimmunoassay for the major structural protein of mason\u2010pfizer monkey virus: Attempts to detect the presence of antigen or antibody in humans"],"prefix":"10.1002","volume":"19","author":[{"given":"Howard P.","family":"Charman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rukhsana","family":"Rahman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Martin H.","family":"White","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nancy","family":"Kim","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raymond V.","family":"Gilden","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(74)90356-0"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910130213"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"1073","DOI":"10.1128\/jvi.19.3.1073-1079.1976","article-title":"Equine infectious anemia virus: evidence favoring classification as a retravirus","volume":"19","author":"Charman H. P.","year":"1976","journal-title":"J. Virol."},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"1409","DOI":"10.1093\/jnci\/52.5.1409","article-title":"Failure to detect in human sera, antibodies cross\u2010reactive with group\u2010specific antigens of murine leukemia virus","volume":"52","author":"Charman H. P.","year":"1974","journal-title":"J. nat. Cancer Inst."},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"1419","DOI":"10.1093\/jnci\/55.6.1419","article-title":"Natural and experimentally induced antibodies to defined mammalian type\u2010C virus proteins in primates","volume":"55","author":"Charman H. P.","year":"1975","journal-title":"J. nat. Cancer Inst."},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1128\/jvi.17.1.51-59.1976","article-title":"Species and interspecies radioimmunoassays for rat type\u2010C virus p30: interviral comparisons and assays of human tumor extracts","volume":"17","author":"Charman H. P.","year":"1976","journal-title":"J. Virol."},{"key":"e_1_2_1_8_1","first-page":"2081","article-title":"A new virus in a spontaneous mammary tumor of a rhesus monkey","volume":"30","author":"Chopra H. C.","year":"1970","journal-title":"Cancer Res."},{"key":"e_1_2_1_9_1","first-page":"11","article-title":"Diagnosis of equine infectious anemia by immunodiffusion test","volume":"33","author":"Coggins L.","year":"1972","journal-title":"Amer. J. vet. Res."},{"key":"e_1_2_1_10_1","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1128\/jvi.17.3.705-712.1976","article-title":"Mason\u2010Pfizer virus RNA genome: relationship to the RNA of morphologically similar isolates and other oncornaviruses","volume":"17","author":"Colcher D.","year":"1976","journal-title":"J. Virol."},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.71.12.4975"},{"key":"e_1_2_1_12_1","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1159\/000149963","article-title":"The case for a family of reverse transcriptase viruses: retraviridae","volume":"4","author":"Dalton A. J.","year":"1974","journal-title":"Intervirology"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1159\/000149440"},{"key":"e_1_2_1_14_1","first-page":"651","article-title":"Responses of infant Rhesus monkeys to inoculation with Mason\u2010Pfizer monkey virus material","volume":"54","author":"Fine D. L.","year":"1975","journal-title":"J. nat. Cancer Inst."},{"key":"e_1_2_1_15_1","unstructured":"Gardner M. B. Murray B. McAllister R. M. Rasheed S. Klement V. Shimizu S. Rongey R. W. Charman H. P. Gilden R. V. Henderson B. E. Heberling R. L. andHuebner R. J. Search for RNA tumor virus in humans.Cold Spring Harbor Conferences on cell proliferation vol. 4 in press (1976)."},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910130212"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1099\/0022-1317-29-3-305"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/257614a0"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)52451-6"},{"key":"e_1_2_1_20_1","first-page":"1323","article-title":"History of a Rhesus monkey adenocarcinoma containing virus particles resembling oncogenic RNA viruses","volume":"48","author":"Mason M. M.","year":"1972","journal-title":"J. nat. Cancer Inst."},{"key":"e_1_2_1_21_1","first-page":"1699","article-title":"A complement\u2010fixation test for the bovine leukemia (C\u2010type) virus","volume":"53","author":"Miller J. M.","year":"1974","journal-title":"J. nat. 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