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When total mononuclear cells or T\u2010lymphocyte\u2010depleted mononuclear cells from the blood of acute IM patients were cultured in the presence of PA at the above concentration, the regular establishment of EB virus genome\u2010containing cell lines seen in control cultures was almost totally abolished. In further experiments, when T\u2010lymphocyte\u2010depleted IM mononuclear cells were co\u2010cultivated with foetal cells of the opposite sex in the presence and absence of PA, cell lines of mixed or of exclusively foetal origin were obtained not only from control co\u2010cultures but also on those rare occasions when transformed foci developed in PA\u2010treated co\u2010cultures. The results suggest that all cell lines derived from the blood of IM patients are initiated in culture by a two\u2010step process of virus release and secondary infection, and argue against the occurrence of any direct outgrowth of IM cells transformed by the virus <jats:italic>in vivo<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/ijc.2910200607","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T17:04:25Z","timestamp":1171904665000},"page":"861-868","source":"Crossref","is-referenced-by-count":26,"title":["Mechanism of the establishment of epstein\u2010barr virus genome\u2010containing lymphoid cell lines from infectious mononucleosis patients: Studies with phosphonoacetate"],"prefix":"10.1002","volume":"20","author":[{"given":"A. 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