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In individual cases more colonies were obtained from blood specimens during the acute phase of the disease than during the convalescent phase. Incorporation of human umbilical cord serum, which contained neutralizing antibody to EBV, into the agar medium did not reduce the number of colonies developing. Our observations indicate that colony\u2010forming cells were originally present in the blood samples, and that they were not infected and subsequently transformed <jats:italic>in vitro.<\/jats:italic> Cells from less than 20% of the EBNA\u2010positive colonies grew to form lymphoblastoid cell lines, which were EBNA\u2010positive and had B lymphocyte surface markers. However, the majority (over 80%) of the EBNA\u2010positive colonies failed to form immortalized cell lines. No colonies were obtained from 91 blood samples from healthy young adults and from five patients with an IM\u2010like disease unrelated to EBV infections. The present results strongly suggest that already transformed cells or cells very easily transformed by EBV are present in the blood of IM patients.<\/jats:p>","DOI":"10.1002\/ijc.2910210405","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T17:01:12Z","timestamp":1171904472000},"page":"426-431","source":"Crossref","is-referenced-by-count":26,"title":["Colonies of ebna\u2010positive cells in soft agar from peripheral leukocytes of infectious mononucleosis patients"],"prefix":"10.1002","volume":"21","author":[{"given":"Yorio","family":"Hinuma","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takato","family":"Katsuki","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"1781","article-title":"Studies of surface immunoglobulins on human B lymphocytes. 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