{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T17:13:05Z","timestamp":1760548385441},"reference-count":24,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2006,7,18]],"date-time":"2006-07-18T00:00:00Z","timestamp":1153180800000},"content-version":"vor","delay-in-days":9895,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1979,6,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>In an attempt to qualitatively identify the membrane antigen (MA) complex induced by Epstein\u2010Barr virus (EBV) infection of lymphoblastoid cells, superinfected Raji cells were surface labelled with <jats:sup>125<\/jats:sup>I by the lactoperoxidase method and solubilized with Triton X\u2010100, then the <jats:sup>125<\/jats:sup>(\u2010labelled membrane proteins were precipitated by sera containing high antibody titers to MA. Analysis of these immune precipitates on sodium dodecyl sulfate polyacrylamide gel electrophoresis identified four major EBV\u2010specific membrane proteins with molecular weights (mol. wt) of 280,000, 250,000, 170,000 and 90,000. Sera from patients with Burkitt's lymphoma (BL), nasopharyngeal carcinoma (NPC) and infectious mononucleosis (IM) and from EBV\u2010infected disease\u2010free individuals showed differential patterns of reactivity to these antigens with some sera only recognizing three or less of the antigens. The results from investigations with these sera also indicated that these major proteins were not related to EBV\u2010induced viral capsid antigens (VCA) or the virus\u2010associated early antigen (EA) complexes as defined by immunofluorescence. Metabolic labelling of EBV\u2010infected Raji cells with [<jats:sup>14<\/jats:sup>C]glucosamine, followed by Triton X\u2010100 solubilization and radioimmune precipitation, identified the 280,000, 250,000 and 90,000 components as glycoproteins. The lactoperoxidase\u2010labelled 170,000 molecular weight component was not significantly glycosylated and, therefore, could not be categorized as a glycoprotein on the basis of this study. In addition, a glycoprotein with a mol. wt of 130,000 was identified by this approach which also appeared to be specified by EBV. The results from these investigations, therefore, indicated that the EBV\u2010induced MA complex was composed of four major glycoproteins and one non\u2010glycosylated high mol. wt protein.<\/jats:p>","DOI":"10.1002\/ijc.2910230612","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T17:45:55Z","timestamp":1171907155000},"page":"808-817","source":"Crossref","is-referenced-by-count":59,"title":["Epstein\u2010Barr virus\u2010induced membrane antigens: Immunochemical characterization of triton X\u2010100 solubilized viral membrane antigens from EBV\u2010superinfected raji cells"],"prefix":"10.1002","volume":"23","author":[{"given":"Louis F.","family":"Qualtiere","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gary R.","family":"Pearson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,18]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1974.tb03599.x"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1093\/jnci\/50.4.841","article-title":"Isolation of plasma membrane antigens from Epstein\u2010Barr virus\u2010infected lymphoid cells","volume":"50","author":"Boone C. W.","year":"1973","journal-title":"J. nat. Cancer Inst."},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj1400345"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"1239","DOI":"10.1093\/jnci\/58.5.1239","article-title":"A solid phase radioimmuno\u2010assay for Epstein\u2010Barr virus\u2010associated membrane antigen prepared from B95\u20108 cell culture supernatant","volume":"58","author":"Dolken G.","year":"1977","journal-title":"J. nat. Cancer Inst."},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"935","DOI":"10.1128\/jvi.17.3.935-949.1976","article-title":"Proteins of Epstein\u2010Barr virus. I. Analysis of the polypeptides of purified enveloped Epstein\u2010Barr virus","volume":"17","author":"Dolyniuk M.","year":"1976","journal-title":"J. Virol."},{"key":"e_1_2_1_7_1","doi-asserted-by":"crossref","first-page":"289","DOI":"10.1128\/jvi.18.1.289-297.1976","article-title":"Proteins of Epstein\u2010Barr virus. II. 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