{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T15:33:50Z","timestamp":1760888030552},"reference-count":27,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":9680,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1980,1,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Under appropriate culture conditions, EB virus infection of lymphocytes from seropositive donors leads to regression of transformation, and this was shown previously to be due to activation in a secondary immune response of T lymphocytes inhibitory for the autologous lymphoblastoid cell line. Regression can be quantified by determining the number of cells required for its expression. To investigate the development of memory T cells with EB\u2010virus specificity in the primary infection, a comparison was made of the capacity for regression of lymphocytes from 16 cases of infectious mononucleosis (IM) and 13 normal donors. With 9 normal seropositive donors a mean lymphocyte concentration of 4.6 \u00d7 10<jats:sup>5<\/jats:sup>\/ml was required to achieve 50% regression. In contrast, with 8 cases of IM tested within I week of onset, a much higher mean lymphocyte concentration (3.7 \u00d7 10<jats:sup>6<\/jats:sup>\/ml) was necessary. Six of these IM cases, and another not tested in the first week, were tested on several occasions between 5 and 23 weeks after onset, and showed a slight reduction in the mean cell concentration required for regression (1.5 \u00d7 1O<jats:sup>6<\/jats:sup>\/ml). Six additional IM cases were tested 23\u201383 weeks after onset by which time the cell concentration required for 50% regression (mean = 4.5 \u00d7 1O<jats:sup>5<\/jats:sup>\/ml) had reached the level shown by normal seropositive donors. Regression did not occur with lymphocytes from seronegative donors, even at the highest cell concentration. Recombination cultures of T\u2010cell\u2010de\u2010pleted and T\u2010cell\u2010enriched lymphocyte populations from 3 IM cases in ratios of 1:7 to 7:1 showed that the failure of regression in acute IM was not due simply to lack of sufficient numbers of T cells. The results indicate that EB\u2010virus\u2010specific memory T\u2010cell activity as detected by the regression test is absent in the acute phase of IM, becomes evident at low levels 5\u201323 weeks after onset, and reaches a maximum after about 6 months.<\/jats:p>","DOI":"10.1002\/ijc.2910250108","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T17:30:37Z","timestamp":1171906237000},"page":"59-65","source":"Crossref","is-referenced-by-count":56,"title":["Long\u2010term T\u2010cell\u2010mediated immunity to epstein\u2010barr virus in man. IV. Development of T\u2010cell memory in convalescent infectious mononucleosis patients"],"prefix":"10.1002","volume":"25","author":[{"given":"A. B.","family":"Rlckinson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. J.","family":"Moss","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. H.","family":"Pope","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N.","family":"Ahlberg","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0304-3835(78)94347-1"},{"issue":"97","key":"e_1_2_1_3_1","first-page":"77","article-title":"Isolation of mononuclear cells and granulocytes from human blood","volume":"21","author":"B\u00f6yum A.","year":"1968","journal-title":"Scand. J. clin. Lab. 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