{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T09:03:28Z","timestamp":1762160608436},"reference-count":17,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,7,18]],"date-time":"2006-07-18T00:00:00Z","timestamp":1153180800000},"content-version":"vor","delay-in-days":9437,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1980,9,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We have investigated the karyotypes and markers of B lymphocyte differentiation in two leukemic cell lines, NALM\u20106\u2010B and NALM\u20106\u2010MI, both derived from a patient with non\u2010T, non\u2010B acute lymphoblastic leukemia. Both possess characteristics of thymus\u2010independent, bursalequivalent (B) lymphocytes. By means of immuno\u2010fluorescence techniques, NALM\u20106\u2010B was shown to possess 47% surface membrane immunoglobulin\u2010positive (Slg<jats:sup>+<\/jats:sup>) cells, while NALM\u20106\u2010MI is surface membrane imunoglobulin\u2010negative (Slg<jats:sup>\u2212<\/jats:sup>), but does possess cytoplasmic immunoglobulin M (ClgM<jats:sup>+<\/jats:sup>) in more than 90% of the cells. The Slg<jats:sup>\u2212<\/jats:sup> ClgM<jats:sup>+<\/jats:sup> phenotype, coupled with morphologic features, is consistent with NALM\u20106\u2010MI being arrested at a stage early in B\u2010lymphocyte differentiation (a \u201cpre\u2010B\u201d cell). The predominant banded chromosome karyotype of NALM\u20106\u2010B is pseudodiploid with a translocation of chromosomal material from the long arm of a chromosome 5 to the short arm of a chromosome 12 (5q<jats:sup>\u2212<\/jats:sup>\/12p<jats:sup>+<\/jats:sup>) and a marker Y chromosome. NALM\u20106\u2010MI possesses the same marker Y chromosome and a deletion of the long arm of chromosome 5 (5q<jats:sup>\u2212<\/jats:sup>) since in most cells material missing from chromosome 5 does not appear on the short arm of chromosome 12. Within the limits of resolution available with the banding technique, no other karyotypic differences are observed in the two cell lines. The history of the two cell lines, the karyotypic analysis, and the pattern of immunoglobulin markers indicate the possible clonal evolution of NALM\u20106\u2010MI from NALM\u20106\u2010B and implicate a portion of the long arm of chromosome 5 in lymphoid differentiation of these leukemic cell lines.<\/jats:p>","DOI":"10.1002\/ijc.2910260305","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T18:01:24Z","timestamp":1171908084000},"page":"281-284","source":"Crossref","is-referenced-by-count":4,"title":["Evolution of cultured leukemic cell lines monitored by chromosomal and immunologic analysis"],"prefix":"10.1002","volume":"26","author":[{"given":"John","family":"Hozier","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Leanna","family":"Lindquist","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard","family":"Hurwitz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tucker","family":"Lebien","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John","family":"Kersey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,18]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"1302","article-title":"Effects of anti mu suppression and cyclophosphamide in pre B cells in mice","volume":"36","author":"Burrows P. D.","year":"1977","journal-title":"Fed. Proc."},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.136.1.191"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(75)90228-4"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI107257"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830071112"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00273497"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910230206"},{"key":"e_1_2_1_9_1","first-page":"689","article-title":"Lymphoid differentiation: an ontogenetic perspective","volume":"10","author":"Lawton A. R.","year":"1978","journal-title":"Transplant. Proc."},{"key":"e_1_2_1_10_1","first-page":"537","article-title":"Chromosomal characteristics and non\u2010random distribution of sister chromatid exchanges in lymphoblastoid cell lines isolated from acute leukemias","volume":"24","author":"Micholova K.","year":"1977","journal-title":"Neoplasma"},{"key":"e_1_2_1_11_1","first-page":"109","article-title":"Human leukemia cell lines: evidence for differentiation toward T\u2010 and B\u2010cell axis within a leukemia","volume":"19","author":"Minowada J.","year":"1978","journal-title":"Proc. Amer. Ass. Cancer Res."},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00293769"},{"key":"e_1_2_1_13_1","first-page":"459","article-title":"Preleukemia: cytogenetic clues to some confusion disorders","volume":"89","author":"Nowel P. C.","year":"1977","journal-title":"Amer. J. Path."},{"key":"e_1_2_1_14_1","first-page":"1294","article-title":"Characterization of precursor B cells in human bone marrow","volume":"36","author":"Okos A. J.","year":"1977","journal-title":"Fed. Proc."},{"key":"e_1_2_1_15_1","doi-asserted-by":"crossref","first-page":"2067","DOI":"10.4049\/jimmunol.118.6.2067","article-title":"Studies on the generation of B lymphocytes in fetal liver and bone marrow","volume":"118","author":"Owen J. J. T.","year":"1977","journal-title":"J. Immunol."},{"key":"e_1_2_1_16_1","doi-asserted-by":"crossref","first-page":"1169","DOI":"10.4049\/jimmunol.120.4.1169","article-title":"B lymphocyte precursors in human bone marrow: an analysis of normal individuals and patients with antibody deficiency states","volume":"120","author":"Pearl E. R.","year":"1978","journal-title":"J. Immunol."},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/259224a0"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(73)93180-2"}],"container-title":["International Journal of Cancer"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fijc.2910260305","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/ijc.2910260305","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,12]],"date-time":"2023-11-12T14:16:57Z","timestamp":1699798617000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/ijc.2910260305"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[1980,9,15]]},"references-count":17,"journal-issue":{"issue":"3","published-print":{"date-parts":[[1980,9,15]]}},"alternative-id":["10.1002\/ijc.2910260305"],"URL":"https:\/\/doi.org\/10.1002\/ijc.2910260305","archive":["Portico"],"relation":{},"ISSN":["0020-7136","1097-0215"],"issn-type":[{"value":"0020-7136","type":"print"},{"value":"1097-0215","type":"electronic"}],"subject":[],"published":{"date-parts":[[1980,9,15]]}}}