{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,23]],"date-time":"2025-10-23T16:36:48Z","timestamp":1761237408279},"reference-count":46,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":9194,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1981,5,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Unfractionated mononuclear (UM) cells and T cells freshly prepared from the blood of adult donors were co\u2010cultivated in microtest plate wells with progressively lower numbers of cells from the autologous EB\u2010virus\u2010transformed B\u2010cell line. The fresh cells present in co\u2010cultures from EB virus antibody\u2010negative (seronegative) donors regularly facilitated autologous cell line outgrowth, monitored after 4 weeks, whereas outgrowth was markedly inhibited in the corresponding co\u2010cultures from seropositive donors. Larger\u2010scale co\u2010cultures, set up at a ratio of 80\u2013100 fresh UM cells to one autologous virus\u2010transformed B cell, were harvested after 8 to 12 days and the T\u2010cell subpopulation was examined for cytotoxicity both by growth inhibition and by chromium release assays. Cytotoxic T cells were generated exclusively in seropositive donor co\u2010cultures and were strongly active against the autologous virus\u2010transformed cell line without affecting either autologous uninfected B cells or any of a range of EB virus genome\u2010negative target cell lines chosen as sensitive indicators of non\u2010specific cytotoxicity. Recognition of allogeneic EB\u2010virus\u2010transformed cells was restricted to those whose HLA\u2010A and\/or B and\/or C antigen expression matched that of the effector cells themselves; moreover target cell lysis was specifically inhibited in the presence of monoclonal antibodies binding to these HLA antigens. The results indicate that EB\u2010virus\u2010specific HLA\u2010restricted memory T cells, present in the blood of previously\u2010infected individuals, can be reactivated <jats:italic>in vitro<\/jats:italic> using the established autologous virus\u2010transformed cell line as a stimulus. The reactivated cytotoxic cells appear to recognize a virus\u2010induced lymphocyte\u2010detected membrane antigen, LYD\u2010MA, analogous to that first invoked to explain the cytotoxic response to primary EB virus infection observed during infectious mononucleosis.<\/jats:p>","DOI":"10.1002\/ijc.2910270505","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T18:13:52Z","timestamp":1171908832000},"page":"593-601","source":"Crossref","is-referenced-by-count":59,"title":["Reactivation of epstein\u2010barr virus\u2010specific cytotoxic t cells by <i>in vitro<\/i> stimulation with the autologous lymphoblastoid cell line"],"prefix":"10.1002","volume":"27","author":[{"given":"A. B.","family":"Rickinson","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. J.","family":"Moss","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. 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