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Histological examination revealed hyperkeratosis, granulosis and moderate acanthosis with large, foamy, basophilic keratinocytes in stratum granulosum and stratum spinosum. Papillomavirus particles could be prepared from these lesions and were differentiated from known papillomavirus types by immune electron microscopy with monospecific antisera and by DNA\u2010DNA hybridization. The viral DNA was characterized by cleavage with several restriction endonucleases and a physical map of the resulting fragments was established. The virus is designated as HPV 8. Preliminary seroepidemiologic studies with human sera indicate a rather wide distribution of HPV 8. Blot hybridization of DNA from human carcinomas with <jats:sup>32<\/jats:sup>P\u2010labelled virus DNA detected no HPV 8\u2010specific sequences.<\/jats:p>","DOI":"10.1002\/ijc.2910270511","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T18:13:52Z","timestamp":1171908832000},"page":"645-650","source":"Crossref","is-referenced-by-count":78,"title":["Characterization of a human papillomavirus from epidermodysplasia verruciformis lesions of a patient from upper\u2010volta"],"prefix":"10.1002","volume":"27","author":[{"given":"Herbert","family":"Pfister","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Friedrich","family":"N\u00fcrnberger","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lutz","family":"Gissmann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harald Zur","family":"Hausen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.201.10.775"},{"key":"e_1_2_1_3_1","first-page":"588","article-title":"Epidermodysplasia verruciformis de Lewandowsky\u2010Lutz chez des Noirs Africains","volume":"16","author":"Bloc G.","year":"1971","journal-title":"Bull. 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