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Immediately thereafter, one group of rats was fed a plain CD diet and the other a choline\u2010supplemented (CS) diet, and subgroups of animals were killed periodically for analysis of the number and size of yGT\u2010positive foci and development of hepatomas. During the first 7 weeks after switching the diets, the number and size of the foci increased in both groups. At 12 and 16 weeks, the number and size of foci began to decline in rats fed the CS diet However, in the CD group, there was a progressive increase in the size with coalescence of the foci, as well as development of neoplastic nodules. These lesions were followed at 20 and 28 weeks by development of hepatomas at a high incidence rate. In rats fed the CS diet, a few scattered yGT\u2010positive foci, and small neoplastic nodules remained in the liver at 20 and 28 weeks, but no hepatomas developed. These results show that the CD diet is a potent promoter of the evolution of foci of altered hepatocytes to hepatomas, and that most of the yGT\u2010positive foci are reversible lesions.<\/jats:p>","DOI":"10.1002\/ijc.2910290414","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T18:41:28Z","timestamp":1171910488000},"page":"445-450","source":"Crossref","is-referenced-by-count":35,"title":["Progression of carcinogen\u2010induced foci of y\u2010glutamyltranspeptidase\u2010positive hepatocytes to hepatomas in rats fed a choline\u2010deficient diet"],"prefix":"10.1002","volume":"29","author":[{"given":"Seiichi","family":"Takahashi","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Benito","family":"Lombardi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hisashi","family":"Shinozuka","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"130","article-title":"Possible mechanism(s) of action of a choline\u2010devoid diet and phenobarbita as promoters of hepatocarcinogenesis","volume":"22","author":"Abanobi S. 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