{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T17:39:52Z","timestamp":1760981992012},"reference-count":21,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":8645,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1982,11,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Three different monoclonal antibodies (mabs) directed against conformation\u2010dependent protein determinants in carcinoembryonic antigen (CEA) were evaluated for tumorimmunolocalization of CEA\u2010containing human tumors grown in nude mice. The antibodies were of high affinity and of the IgG<jats:sub>1<\/jats:sub> subclass. They did not react with non\u2010specific cross\u2010reacting antigen (NCA), biliary glyco\u2010protein 1 (BGP 1) or orosomucoid. 10\u201320 \u03bcCi(1\u20132 \u03bcg) of <jats:sup>131<\/jats:sup>I\u2010labelled mab was injected intraperitoneally into mice bearing heterografts of the CEA\u2010containing human tumor cell lines LS 174T, Detroit 562 or HT\u201029 or into mice bearing grafts of the non\u2010CEA\u2010containing human tumor cell line RD. The concentration of radiolabelled mab in the tumor compared with the mean concentration in the mouse increased up to day 4 after injection. In contrast, the concentration ratios of radioactivity in the other tissues decreased with time. Excellent tumor localization was obtained with mab 38SI in mice bearing LS 174T tumors. The concentration of radioactivity (cpm\/g) tissue wet weight) was 24\u2013390 times higher in the tumor than in the muscle. The difference between tumor and liver and between tumor and blood was 4\u201389 times and 2\u201325 times, respectively. A mean value of 40% of the total radioactivity in the mouse at day 4 was localized to the tumor. Mab 38SI also showed positive tumor localization in mice carrying Detroit and HT\u201029 tumors. Means of 22% and 18% of the total radioactivity in the mouse at day 4 were found in these tumors, respectively. Mab 38SI was, however, not enriched in the tumor of mice bearing the non\u2010CEA\u2010containing sarcoma RD. Furthermore, when LS 174T tumor\u2010bearing mice were injected with normal mouse IgG, no enrichment of radioactivity in the tumor was seen. Positive tumor localization with mab 48 was obtained while mab 9 was not enriched in the tumor. Scanning pictures of LS 174T\u2010grafted mice on day 4 after injection of <jats:sup>131<\/jats:sup>I\u2010mab 38SI showed a definite radioactive spot corresponding to the tumor and in addition a spot corresponding to the liver or the heart.<\/jats:p>","DOI":"10.1002\/ijc.2910300503","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T19:15:05Z","timestamp":1171912505000},"page":"547-552","source":"Crossref","is-referenced-by-count":50,"title":["Tumor localization of CEA\u2010containing human tumors in nude mice by means of monoclonal anti\u2010CEA antibodies"],"prefix":"10.1002","volume":"30","author":[{"given":"Anders","family":"Hedin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Britta","family":"Wahren","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sten","family":"Hammarstr\u00f6m","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.280.6209.220"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/0161-5890(78)90070-6"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4757-1647-4"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197806222982503"},{"key":"e_1_2_1_6_1","first-page":"2984","article-title":"Radioimmunodetection of cancer with radioactive antibodies to carcinoembryonic antigen","volume":"40","author":"Goldenberg D.","year":"1980","journal-title":"Cancer Res."},{"key":"e_1_2_1_7_1","first-page":"1","article-title":"Photoscan localization of GW\u201039 tumors in hamsters using radiolabelled anticarcinoembryonic antigen immunoglobulin G","volume":"34","author":"Goldenberg D.","year":"1974","journal-title":"Cancer Res."},{"key":"e_1_2_1_8_1","doi-asserted-by":"crossref","unstructured":"Hedin A. Hammarstr\u00f6m S. andLarsson \u00c5. Specificities and binding properties of eight monoclonal antibodies against carcinoembryonic antigen.Mol. Immunol.(1982) (in press).","DOI":"10.1016\/B978-0-08-027988-6.50210-X"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.76.3.1438"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/194495a0"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/0010-4825(81)90006-8"},{"key":"e_1_2_1_12_1","first-page":"3526","article-title":"Cell surface alterations on colon adenocarcinoma cells","volume":"36","author":"Kahan B.","year":"1976","journal-title":"Cancer Res."},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.2307\/3001469"},{"key":"e_1_2_1_14_1","first-page":"570","article-title":"Localization of I\u2010131 labelled tumor\u2010specific monoclonal antibody in the tumor\u2010bearing BALB\/c mouse","volume":"21","author":"Levine G.","year":"1980","journal-title":"J. nucl. 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