{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,3]],"date-time":"2025-11-03T09:03:14Z","timestamp":1762160594939},"reference-count":36,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,7,18]],"date-time":"2006-07-18T00:00:00Z","timestamp":1153180800000},"content-version":"vor","delay-in-days":8404,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1983,7,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Peripheral blood T cells from a patient with T\u2010cell chronic lymphocytic leukemia (T\u2010CLL) failed to respond to mitogenic lectins or alloantigens but could be induced to proliferate by the addition of exogenous interleukin\u20102 (IL\u20102). The T\u2010CLL cells also proliferated in response to 12\u2010<jats:italic>O<\/jats:italic>\u2010tetradecanoyl phorbol\u201013\u2010acetate (TPA), or to TPA in combination with phytohemagglutinin (PHA). These TPA\u2010mediated proliferative responses were transient, and only small but significant amounts of IL\u20102 activity were generated. In contrast, no IL\u20102 activity was produced after the T\u2010CLL cells had been stimulated with PHA only. The T\u2010CLL cells that were induced to proliferate with PHA and exogenous IL\u20102 could be maintained in continous culture by the addition of exogenous IL\u20102 at regular intervals. These continuously proliferating T\u2010CLL cells failed to produce IL\u20102 constitutively. However, they could be induced to produce IL\u20102 activity by stimulation with TPA or TPA plus PHA. Irradiation of the proliferating T\u2010CLL cells prior to incubation with TPA or TPA plus PHA resulted in a 9\u2010fold increase in IL\u20102 activity, suggesting that the proliferating T\u2010CLL cells were able to consume the IL\u20102 they produced. Studies on the presence of human T\u2010cell leukemia\/lymphoma virus (HTLV) in the fresh and proliferating T\u2010CLL cells revealed that 12% of the fresh cells expressed the HTLV p19 structural core protein. HTLV p19 expression was strongly enhanced in the T\u2010CLL cells induced to proliferate by TPA (66%) and in the continuously growing IL\u20102\u2010dependent T\u2010CLL cells (82%). In the latter culture, but not in the fresh T\u2010CLL cells, type\u2010C virus particles were observed. These results indicate that HTLV expression correlates with T\u2010CLL cell proliferation but not with IL\u20102 production by these cells.<\/jats:p>","DOI":"10.1002\/ijc.2910320109","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T19:32:53Z","timestamp":1171913573000},"page":"53-59","source":"Crossref","is-referenced-by-count":13,"title":["Enhanced expression of human T\u2010cell leukemia\/lymphoma virus in neoplastic T cells induced to proliferate by phorbol ester and interleukin\u20102"],"prefix":"10.1002","volume":"32","author":[{"given":"Florry A.","family":"Vyth\u2010Dreese","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jan E.","family":"De Vries","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,18]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"406","article-title":"Detection of type C virus particles in short\u2010term cultured adult T\u2010cell leukemia cells","volume":"47","author":"Akagi T.","year":"1982","journal-title":"Lab. 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