{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T23:30:28Z","timestamp":1697844628534},"reference-count":25,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,7,19]],"date-time":"2006-07-19T00:00:00Z","timestamp":1153267200000},"content-version":"vor","delay-in-days":8313,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1983,10,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A highly immunogenic variant of the murine L1210 leukemia cell (L1210\/LN\u20101) for generation of tumor immunity has been obtained from culture of L1210 cells originally fused with human Lesch\u2010Nyhan fibroblasts. In L1210\/LN\u20101 cells, no human chromosomes were identified and chromosomes Ml and No. I carried by the parent L1210 cells were missing. L1210\/LN\u20101 cells displayed an intermediate morphology between L1210 cells and Lesch\u2010Nyhan fibroblasts. Most of the CDF<jats:sub>1<\/jats:sub> mice that were inoculated with less than 2 \u00d7 10<jats:sup>6<\/jats:sup> L1210\/LN\u20101 cells survived over 60 days without evidence of tumor, whereas the original L1210 cells killed all the mice tested in about 2 weeks. When inoculated with more than 5 \u00d7 10<jats:sup>6<\/jats:sup> L1210\/LN\u20101 cells, CDF<jats:sub>1<\/jats:sub> mice developed tumor. The CDF<jats:sub>1<\/jats:sub> mice which rejeted 2 \u00d7 10<jats:sup>6<\/jats:sup> L1210\/LN\u20101 cells were protected very effectively against challenge of otherwise highly aggressive 1\u20135 \u00d7 10<jats:sup>5<\/jats:sup> L1210 leukemia cells; 40 out of 43 primed mice tested survived for 60 days or longer after the tumor challenge. Even the CDF<jats:sub>1<\/jats:sub> mice primed with irradiated or mitomycin\u2010treated L1210\/LN\u20101 cells survived against a challenge of 10<jats:sup>s<\/jats:sup> L1210 leukemia cells. They were, however, not protected against P388 leukemia or Meth A sarcoma, indicating that the immunity was specific to L1210 leukemia. The immunity induced by L1210\/LN\u20101 cells was transplantable by immune spleen cells into syngeneic recipients. Thus, the L1210\/LN\u20101 cells we obtained seem to be very useful as an immunogen for generation of high\u2010grade tumor\u2010specific immunity against highly malignant L1210 leukemia.<\/jats:p>","DOI":"10.1002\/ijc.2910320419","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T19:28:39Z","timestamp":1171913319000},"page":"507-514","source":"Crossref","is-referenced-by-count":6,"title":["High\u2010grade tumor\u2010specific immunity induced by L1210 leukemia variants obtained from the culture of L1210 cells fused with Lesch\u2010Nyhan fibroblasts"],"prefix":"10.1002","volume":"32","author":[{"given":"K.","family":"Kawashima","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Nagura","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E.","family":"Watanabe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Mizoguchi","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Saga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Isobe","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"I.","family":"Nakashima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Yamada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"T.","family":"Oikawa","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Kojima","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,19]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"2598","article-title":"Protective effect of an interspecies hybridoma on the tumorigenicity of mouse myeloma cells","volume":"41","author":"Baumal R.","year":"1981","journal-title":"Cancer Res."},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.74.1.272"},{"key":"e_1_2_1_4_1","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1093\/jnci\/65.4.739","article-title":"Induced resistance in normal mice and growth enhancement in tumor\u2010bearing mice by inoculation with syngeneic hybrid cells","volume":"65","author":"Dei T.","year":"1980","journal-title":"J. nat. 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