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The lines were established from T\u2010cell leukemia\u2010lymphoma patients or HTLV\u20101\u2010transformed human umbilical cord\u2010blood T cells. Clumps of transformed cells became visible by 2\u20133 weeks after infection and developed at a high frequency (&gt;90%) in the bone marrow samples used. Stimulation of target cells with lymphocyte mitogens facilitated this process but was not absolutely required. Unlike fresh or cultured cells from HTLV\u20101\u2010positive adult leukemia\u2010lymphoma patients and HTLV\u20101\u2010transformed cord\u2010blood T cells, which usually have an OKT 4\/leu 3a surface phenotype, the transformed bone marrow cells frequently fell into one of three categories based on their reactivity with cell\u2010specific monoclonal antibodies. These included populations of cells that were predominantly; (1) OKT 4\/leu 3a\u2010positive, (2) OKT 8\/leu 2a\u2010positive, and (3) cells expressing neither phenotype. Fresh bone marrow provided a rapid and efficient system for the assessment of HTLV\u20101 infection. The type of bone marrow cells transformed <jats:italic>in vitro<\/jats:italic> suggests that HTLV\u20101 can infect several subsets of T lymphocytes, possibly including immature cells or that these cells can undergo phenotypic modulation.<\/jats:p>","DOI":"10.1002\/ijc.2910330104","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T19:23:53Z","timestamp":1171913033000},"page":"13-17","source":"Crossref","is-referenced-by-count":53,"title":["Transformation of different phenotypic types of human bone marrow T\u2010lymphocytes by htlv\u20101"],"prefix":"10.1002","volume":"33","author":[{"given":"Phillip D.","family":"Markham","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S. 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