{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T06:42:19Z","timestamp":1791096139477,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":8006,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1984,8,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Human T\u2010cell leukemia\/lymphoma virus (HTLV)\u2010carrying cells from various origins were characterized by cell surface markers and expression of HTLV antigens. Eight cell lines named TCL were obtained by transformation of peripheral blood leukocytes (PBL) of healthy donors or HTLV carriers in cocultures with HTLV\u2010producer MT\u20102 cells. Nine cell lines named ILT were interleukin 2 (IL2)\u2010dependent cell lines cloned from PBL of ATL patients and healthy HTLV\u2010carriers. Tc\u2010Kan9 cell line was also an IL2\u2010dependent cell line clonally established from PBL culture stimulated with autologous TCLcells. Five cell lines named TL were established <jats:italic>in vitro<\/jats:italic> directly from PBL of an adult T\u2010cell leukemia (ATL) patient and from ILT cells of an ATL patient and three HTLV\u2010carriers, respectively, to grow autonomously without IL2. All the TCLs, ILTs, TLs and Tc\u2010Kan9 possessed Leu\u2010I antigen, a pan\u2010T\u2010cell marker. Leu3a antigen, a helper\/inducer T\u2010cell marker, was expressed on five of eight TCLs and all of the ILTs and TLs. Leu\u20102a, a cytotoxic\/suppressor T\u2010cell marker, was detected only on Tc\u2010Kan9 but not others. Fresh ATL leukemic cells of patients had a helper\/inducer T\u2010cell marker. Ia, OKT9 and Tac antigens, markers for activated and differentiated T cells, were strongly expressed on all of the cell lines tested and fresh ATL leukemic cells were weakly positive for these antigens. Expression of HTLV antigens detected by mouse monoclonal antibodies and an ATL\u2010patient serum varied among these cell lines. One TL, two ILTs and most of the fresh ATL leukemic cells did not express HTLV antigens on the cell surface. The other cell lines were all positive for the surface viral antigens. However, molecular species of antigens defined by radioimmunoprecipitation with an ATL\u2010patient serum were not always identical among the cell lines. Molecular weights of polypeptides detectable in most of the cell lines were 62K, 46K, 40K, 24K, 21K and 19K which could never be detected in several control T\u2010cell lines. 68K and 28K polypeptides were frequently detected in MT\u20102 and TCLs. GINI4, a mouse monoclonal antibody against HTLV core protein (p19) detected not only p19 in various cell lines but also p28, p29, p31 or p40 in certain cell lines tested. B\u2010cell lines named LCL were established and cloned from PBL of two HTLV\u2010carriers by EB\u2010virus\u2010induced transformation and they also expressed HTLV antigens, la, OKT9 and Tac antigens. Expression of Tac and HTLV antigens of fresh ATL leukemic cells were induced or enhanced after <jats:italic>in vitro<\/jats:italic> short\u2010term cell cultivation with crude IL2.<\/jats:p>","DOI":"10.1002\/ijc.2910340213","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T19:44:38Z","timestamp":1171914278000},"page":"221-228","source":"Crossref","is-referenced-by-count":149,"title":["Cell surface phenotypes and expression of viral antigens of various human cell lines carrying human T\u2010cell leukemia virus"],"prefix":"10.1002","volume":"34","author":[{"given":"Kazuo","family":"Sugamura","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masahiro","family":"Fujii","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mari","family":"Kannagi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitsuru","family":"Sakitani","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masao","family":"Takeuchi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yorio","family":"Hinuma","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V62.2.401.401"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(196504)18:4<522::AID-CNCR2820180418>3.0.CO;2-J"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.79.15.4780"},{"key":"e_1_2_1_5_1","first-page":"790","article-title":"Identification of envelope glycoprotein encoded by env gene of human T\u2010cell leukemia virus","volume":"74","author":"Hattori T.","year":"1983","journal-title":"Gann"},{"key":"e_1_2_1_6_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V58.3.645.645"},{"key":"e_1_2_1_7_1","first-page":"99","article-title":"Transmission of adult T\u2010cell leukemia virus from lymphoid cells to non\u2010lymphoid cells associated with cell membrane fusion","volume":"75","author":"Hayami M.","year":"1984","journal-title":"Gann"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.157.3.907"},{"key":"e_1_2_1_9_1","first-page":"211","article-title":"Association of a retrovirus (ATLV) with adult T\u2010cell leukemia: review of serologic studies","volume":"28","author":"Hinuma Y.","year":"1982","journal-title":"Gann Monogr. 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