{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T17:11:06Z","timestamp":1697908266503},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":7959,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1984,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Clonogenic cells from peripheral blood of 13\/16 patients with T\u2010cell malignancies generated colonies in methylcellulose in absence of added growth factors or mitogenic stimulation. Spontaneous colonies were also obtained from purified cell fractions (E<jats:sup>\u2212<\/jats:sup>OKT3<jats:sup>\u2212<\/jats:sup> and\/or E<jats:sup>+<\/jats:sup> cells) in 73% and 57% of the patients, respectively. No spontaneous colony growth was observed with mononuclear cells of patients with solid tumors, non\u2010T\u2010cell leukemias or normal subjects. Colonies consisted of acid\u2010phosphatase\u2010positive, myeloperoxidase\u2010and PAS\u2010negative lymphoblasts bearing T\u2010cell surface markers. Although the phenotype of pooled colony cells from either unfractionated mononuclear cells or E<jats:sup>\u2212<\/jats:sup>OKT3<jats:sup>\u2212<\/jats:sup>\u2010derived colonies varied from patient to patient, the colonies, like fresh leukemic cells, were mostly composed of relatively immature cells as assessed by the high proportion (&gt;40%) of OKT6<jats:sup>+<\/jats:sup> and OKT10<jats:sup>+<\/jats:sup> cells and the low proportion (&lt;40%) of OKT3<jats:sup>+<\/jats:sup> and\/or E<jats:sup>+<\/jats:sup> cells. Cytogenetic analysis of colony cells revealed either normal metaphases or chromosome anomalies similar to those observed in fresh leukemic cells. Moreover, cells from primary colonies exhibited a capacity for self\u2010renewal in the absence of added growth factors.<\/jats:p>","DOI":"10.1002\/ijc.2910340407","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T19:40:47Z","timestamp":1171914047000},"page":"471-477","source":"Crossref","is-referenced-by-count":7,"title":["Colony formation in the absence of added growth factors by peripheral blood T\u2010cell colony\u2010forming cells of patients with T\u2010cell malignancies"],"prefix":"10.1002","volume":"34","author":[{"given":"Vassilis","family":"Georgoulias","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aldar","family":"Bourinbaiar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fran\u00e7oise","family":"Amesland","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chantal","family":"Canon","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huguette","family":"Auclair","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claude","family":"Jasmin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"526","article-title":"Colony formation by human lymphocytes in agar medium","volume":"28","author":"Cla\u00ebsson M. 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