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Both antibodies showed complement\u2010dependent cytotoxicity against KMT\u201017. By absorption of cytotoxicity, KH\u20101 reacted with homologous tumor, other syngeneic fibrosarcomas (KMT\u201080 and KMT\u201075), and lung and kidney from normal rats. However, KH\u20102 reacted with many kinds of tumors and various normal tissues. Antigen specificity was tested by complement fixation and\/or solidphase radioimmunoassay using glycolipids isolated from KMT\u201017 cells and authentic glycolipids. KH\u20101 reacted with globotriglycosyl ceramide which was not detected on KMT\u201017 cells and in cross\u2010reacted weakly with IV<jats:sup>3<\/jats:sup>\u2010\u03b1\u2010galactosyl\u2010lactoneotetraglycosyl ceramide, one of the major glycolipids of KMT\u201017. The immune reaction was inhibited by \u03b1\u2010methyl\u2010galactose. KH\u20102 reacted with lactosyl ceramide and lactoneotetraglycosyl ceramide. The reaction was more potently inhibited by lactose than by \u03b2\u2010methyl\u2010galactose. Antibodies with similar specificity to either KH\u20101 or KH\u20102 were elevated in syngeneic rat sera after serial immunization with viable KMT\u201017 cells.<\/jats:p>","DOI":"10.1002\/ijc.2910340517","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T19:40:19Z","timestamp":1171914019000},"page":"689-697","source":"Crossref","is-referenced-by-count":29,"title":["Carbohydrates as antigenic determinants of tumor\u2010associated antigens recognized by monoclonal anti\u2010tumor antibodies produced in a syngeneic system"],"prefix":"10.1002","volume":"34","author":[{"given":"Masafumi","family":"Ito","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eiji","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masaharu","family":"Naiki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fujiro","family":"Sendo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeru","family":"Arai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"crossref","first-page":"335","DOI":"10.1093\/oxfordjournals.jbchem.a129645","article-title":"Application of trifluoroacetyl derivatives to sugar and lipid chemistry. 1. 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