{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,11,21]],"date-time":"2023-11-21T06:46:38Z","timestamp":1700549198776},"reference-count":35,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":7794,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1985,3,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The Epstein\u2010Barr virus\u2010determined nuclear antigen (EBNA) is the only known virally\u2010determined compoment that is regularly associated with EBV\u2010transformed cells. A main component of EBNA, herein designated EBNA\u20101, has been conclusively localized to the BamHI K fragment of the viral genome. EBNA\u20101 is present in all EBV carrying cell lines so far studied. Our current study deals with a second component. We have found that the EBNA reaction detected by anticomplement immunofluorescence (ACIF) in Burkitt lymphoma lines Daudi, Jijoye, and P3HR\u20101 could be completely removed by preabsorption of sera with any one of these 3 lines, when tested against any other of them. The same absorbed sera still gave a brilliant nuclear staining against other EBV\u2010carrying lines, e. g. Raji or B95\u20108. The 3 lines in the first category carry EBV genomes that have delections in the BamHI WYH region of the EBV genome. This region is intact in the second group of lines. This result is interpreted as showing the existence of 2 different ACIF\u2010stainable EBV\u2010determined nuclear antigens, one of which is associated with the BamHI WYH region. We designate this antigen as EBNA\u20102. We found that the two different EBNAs are different with regard to their association with metaphase chromosomes. In lines positive for both EBNA subtypes, metaphase chromosomes gave brilliant EBNA\u20101 staining, but could not be stained for EBNA\u20102, indicating differences in chromatin association of the two EBNAs. An 86 kd polypeptide was identified by immunoblotting of DNA\u2010binding proteins from EBV\u2010transformed lymphoid cell lines. EBV\u2010specificity of the polypeptide was demonstrated by the presence of antibodies against this polypeptide in antisera from a population of EBV\u2010seropositive donors, but not from seronegative donors, by the presence of the polypeptide itself in EBV\u2010carrying but not in EBV\u2010negative cell lines and by the appearance of antibodies against this polypeptide during the course of infectious mononucleosis (IM). The polypeptide was absent from the EBV\u2010carrying P3HR\u20101, Daudi and Jijoye cell lines, which suggested that it may be encoded by the BamHI WYH region that is deleted from the viral substrains carried by these lines.<\/jats:p>","DOI":"10.1002\/ijc.2910350312","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T20:15:44Z","timestamp":1171916144000},"page":"359-366","source":"Crossref","is-referenced-by-count":41,"title":["Characterization of a second Epstein\u2010Barr virus\u2010determined nuclear antigen associated with the BamHI WYH region of EBV DNA"],"prefix":"10.1002","volume":"35","author":[{"given":"Joakim","family":"Dillner","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bengt","family":"Kallin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Barbro","family":"Ehlin\u2010Henriksson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Laszlo","family":"Timar","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George","family":"Klein","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,17]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0076-6879(71)21013-2"},{"key":"e_1_2_1_3_1","doi-asserted-by":"crossref","first-page":"376","DOI":"10.1128\/jvi.41.2.376-389.1982","article-title":"Characterization of the major Epstein\u2010Barr virus\u2010Specific RNA in Burkitt lymphoma\u2010Derived cells","volume":"41","author":"Arrand J. R.","year":"1982","journal-title":"J. Virol."},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/310207a0"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"2875","DOI":"10.1016\/S0021-9258(17)40903-3","article-title":"Partial purification and properties of the Epstein\u2010Barr virus associated nuclear antigen","volume":"253","author":"Baron D.","year":"1978","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"503","DOI":"10.1128\/jvi.35.3.603-618.1980","article-title":"Comparison of Epstein\u2010Barr virus strains of different origin by analysis of the viral DNAs","volume":"35","author":"Bornkamm G. W.","year":"1980","journal-title":"J. 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USA in press."},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.78.3.1930"},{"key":"e_1_2_1_34_1","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1128\/jvi.38.3.996-1004.1981","article-title":"Identification of an Epstein\u2010Barr virus nuclear antigen by fluoroimmunoelectrophoresis and radioimmunoclectrophoresis","volume":"38","author":"Strnad B.","year":"1981","journal-title":"J. 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