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In this group HPV\u2010DNA was detected in 5 cases (2.2%).<\/jats:p><jats:p>Of 82 cytologically positive cases (25 confirmed by histology) 56 (68%) contained HPV\u2010DNA. A high prevalence of HPV 6\/11 and absence of HPV 16\/18 was found in cases with cytological signs of permissive HPV infection. In mild and moderate dysplasia all viruses occurred at almost the same frequency. In severe dysplasia\/carcinoma <jats:italic>in situ<\/jats:italic> HPV 16\/18 was found 5 times more frequently than HPV 6\/11. HPV 16\/18 was identified in all 4 invasive cancer cases.<\/jats:p><jats:p>Cervical irrigation of colposcopically suspect areas was performed in 15 cytologically and HPV\u2010DNA positive cases using the hydrodynamic filtration method. In 12 cases only the cells obtained from the colposcopically positive areas contained HPV\u2010DNA.<\/jats:p><jats:p>The sensitivity and reproducibility of the filter <jats:italic>in situ<\/jats:italic> hybridization was shown by: (1) comparing the results obtained by HPV\u2010DNA hybridization using Southern blot analysis of tumor biopsies; (2) analysing the correlation of cytologic diagnosis and presence of HPV\u2010DNA in follow\u2010up examinations, and (3) diagnosing presence or absence of HPV\u2010DNA in parallel filters from the same patients.<\/jats:p>","DOI":"10.1002\/ijc.2910350405","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T20:14:07Z","timestamp":1171916047000},"page":"443-448","source":"Crossref","is-referenced-by-count":141,"title":["Papillomavirus infection of the lower genital tract: Detection of viral DNA in gynecological swabs"],"prefix":"10.1002","volume":"35","author":[{"given":"Achim","family":"Schneider","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Herbert","family":"Kraus","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Roland","family":"Schuhmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lutz","family":"Gissmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,7,18]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"509","article-title":"Detection of cervical cancer in a women's prison","volume":"104","author":"Audet\u2010LaPointe P.","year":"1971","journal-title":"Can. med. 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