{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T05:40:19Z","timestamp":1697866819139},"reference-count":39,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2006,7,17]],"date-time":"2006-07-17T00:00:00Z","timestamp":1153094400000},"content-version":"vor","delay-in-days":7702,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Intl Journal of Cancer"],"published-print":{"date-parts":[[1985,6,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The extracellular calcium level required for proliferation was compared in B lymphoid cell lines from various sources by determining the calcium concentration at which long\u2010term proliferation was inhibited by 50% (CaPD<jats:sub>50<\/jats:sub>). Fourteen Burkitt lymphoma (BL) lines had a mean CaPD<jats:sub>50<\/jats:sub> of 44 \u00b1 28 \u03bcM whereas 45 lymphoblastoid cell lines (LCLs) obtained by <jats:italic>in vitro<\/jats:italic> transformation of B lymphocytes with Epstein\u2010Barr virus (EBV) had a mean CaPD<jats:sub>50<\/jats:sub> of 3.6 \u00b1 1.8 \u03bcM. This difference applied also to autologous BL lines and LCLs established from the same patient. The decreased calcium requirement of virally\u2010transformed compared with tumour\u2010derived cell lines therefore appears to be a universal phenomenon in mamalian cells. Within the BL group, no correlation was found between the calcium requirement for proliferation and presence or absence of the EBV genome. Arrest of BL lines and LCLs occurred in the G<jats:sub>1<\/jats:sub> phase of the cell cycle and was readily reversed by additional of calcium to the medium. One anomalous LCL was found which showed a high CaPD<jats:sub>50<\/jats:sub> (43 \u00b1 6 \u03bcM) and accumulated in both G<jats:sub>1<\/jats:sub> and G<jats:sub>2<\/jats:sub>. These results, in combination with a previous study of EBV transformation <jats:italic>in vitro<\/jats:italic>, indicate that the calcium dependence of B lymphocytes generally decreases in the following order: normal cells &gt; BL cells = early stage transformation &gt; LCL. The 2 transformed phenotypes thus distinguished in human lymphoid cells may offer unique opportunities for defining the status and expression of EBV <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic>.<\/jats:p>","DOI":"10.1002\/ijc.2910350608","type":"journal-article","created":{"date-parts":[[2007,2,19]],"date-time":"2007-02-19T20:12:55Z","timestamp":1171915975000},"page":"743-747","source":"Crossref","is-referenced-by-count":0,"title":["Decreased calcium dependence of lymphoblastoid cell lines compared with burkitt lymphoma cell lines"],"prefix":"10.1002","volume":"35","author":[{"given":"P. G.","family":"Parsons","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"D. 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